We narrowed to 779 results for: mos

1. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...day old human blastocyst [6]. They are isolated mostly from abnormal and/or supernumerary preimplantation... generate specialized cells for their location, mostly to maintain cell numbers or replenish dying cells...

2. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...that forms chromosomes. These long range DNA interactions are often detected with chromosome conformation...

3. Cloning Mammalian Cells with the Agarose Method

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   ...cell lines, especially the endocrine cell lines we most commonly work with, require extra serum to survive... of the wells in the 48-well plate will grow up, most will eventually grow within a week. We screen the...

4. Truncated gRNAs for Regulating Gene Expression

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   ...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...

5. Plasmids 101: Knockout/Knock-In Plasmids

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   ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

6. Google Forums Round Up: First Impressions of NgAgo

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   ...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...

7. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...common issues follow. It's a matter of concentration Most FPs in nature are prone to dimerization (i.e. EGFP... FPs such as TagRFP. Problem 3 can be solved, in most cases, by using monomeric FPs or FPs from different...

8. Light Sheet Fluorescence Microscopy

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   ...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

9. Plasmids 101: Multicistronic Vectors

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   ...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

10. Tips for Using BLAST to Verify Plasmids

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    ...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

11. Antibodies 101: Producing Recombinant Antibodies

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    ...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

12. Getting to Know Expansion Microscopy

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    ...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ... Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses. I could...inspiration for our updated mascot, it’s also a convenient most requested filter. Applying a second filter, this...

14. SciComm: Taking Science to Elementary and Middle Schools

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    ...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

15. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

16. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company... atmosphere...

17. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ...every biologist, academic, and researcher. Among the most well-known CRISPR-associated nucleases are Cas9 ...just 408 amino acids long — making it one of the most compact genome editors to date. We have developed...

18. QC Sequencing Technologies at Addgene

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    ...this technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have ...

19. Hot Plasmids: Fall 2024

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    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...

20. Tips for Writing a Good Cover Letter

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    ...field of research], and have excellent [list the most important skills for the position]. I am enthusiastic... does not need to be a literary masterpiece. For most jobs, the writing quality required will be close...