We narrowed to 775 results for: mos

... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...

...process of even applying to one of these programs is almost as daunting as the program itself. Picking a mentor...idea to email around three professors per program. Most applications will recommend that you name two professors...

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

... Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses. I could...inspiration for our updated mascot, it’s also a convenient most requested filter. Applying a second filter, this...

...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

...’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company... atmosphere...

...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

...communicate your story with only the most salient details. Make the most of your limited speaking time. Beyond...

...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...

...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...

...field of research], and have excellent [list the most important skills for the position]. I am enthusiastic... does not need to be a literary masterpiece. For most jobs, the writing quality required will be close...