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  3. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology park...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  4. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27). Joined...

  5. The Materials Science of Optogenetics Experiments

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    Blog Post
    ... are all wired up and expressing your opsin of interest, it’s time to get to the fun stuff: the experiments...Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory in opioid...

  6. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors to VP1,...into a compartment encapsulating the protein of interest (Figure 3).   Figure 3: Schematic representation...

  7. Viral Vectors 101: Voltage Indicators

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    Blog Post
    ...selected to preferentially target your cell type of interest. Viral pseudotyping is also a powerful tool that... optimize them for targeting your cell type of interest. For in vivo imaging applications, the ability...

  9. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left...Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich Introduction...

  10. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...localization of the FP (fused to the protein of interest), thus tracking the localization of that biomolecule...at Albert Einstein College of Medicine. He is interested in everything related to gene expression, particularly...

  13. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance... retained a functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics...

  14. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post
    ...biologists working with vectors are, in most cases, interested in transferring genes to mammalian cells (i.e... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

  15. Great Results Start with Great Standard Curves

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    Blog Post
    ...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if...

  16. AAVs in Retinal Gene Therapy

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    Blog Post
    ...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...

  17. Plasmids 101: Colony PCR

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    Blog Post
    ...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope proteins from another virus to either restrict or broaden the host cell range (tropism). Pseudotyping..., Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...scientists can make mutations throughout a gene of interest based on an organisms codon usage bias to increase...when do you want to codon optimize your gene of interest? Codon optimization has become extremely useful...
Showing: 481 - 500 of 755 results