We narrowed to 610 results for: sam

1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...confirmed the insertion of the Lambda insert at the same high frequencies as the antibiotic selection markers...

2. Plasmids 101: Protein Expression

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   ...protein expression. Plant systems share many of the same features and processing requirements as mammalian...

3. Truncated gRNAs for Regulating Gene Expression

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   ...2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for genome editing...

4. Plasmids 101: Knockout/Knock-In Plasmids

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   ...one of its exons has simply been replaced with the same sequence flanked by LoxP sites while the resistance...

5. Google Forums Round Up: First Impressions of NgAgo

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   ... to deliver NgAgo ingredients into the cell, the same method used by Gao et al, 2016 (1). View the Complete...

6. Genome-wide Screening Using CRISPR

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   ...Enrichment or depletion of multiple gRNAs targeting the same gene can be strong evidence that a particular gene...

7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ... with a knockout and expression construct on the same piece of DNA. We do a lot of protein-protein interaction...

8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...BioBricks Foundation to distribute the physical DNA samples, for when that's the preferred mode of receiving...

9. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ... Central PMCID: 5088052. 3. Lentz TB, Gray SJ, Samulski RJ. Viral Vectors for Gene Delivery to the Central...

10. SciComm: Taking Science to Elementary and Middle Schools

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    ...essentially background slides getting everyone on the same page in terms of the vocabulary--DNA, protein, plasmid...

11. Antibodies 101: Producing Recombinant Antibodies

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    ....2018.08.043. Epub 2018 Aug 23. PMID: 30145245. Samiul Amin, Gregory V. Barnett, Jai A. Pathak, Christopher...

12. CRISPR 101: RNA Editing with Cas13

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    ...delivery. They found C-terminal truncations allowed the same or improved editing ability in a RESCUE system small...

13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...interpretation -- the effect would generally not be the same if weekends were somehow imposed on scientists. ...

14. Course-Based Undergraduate Research Experiences (CUREs)

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    ...happened in the lab, and he wanted to provide the same kinds of opportunities for his students. But he ...

15. Summer SciComm Series: Modes of Communication

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    ... allow people to learn in multiple ways from the same experience.  Can combines cons of multiple medias...

16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...technically possible to encode all four viral genes in the same plasmid (as opposed to four separate plasmids), ...

17. Cpf1: A New Tool for CRISPR Genome Editing

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    ...cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct repeat preceding...

18. Plasmids 101: Protein tags

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    ...protein since the enzyme cleaves the SUMO tag in the same manner as it would have in the context of a cell...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...grant applications, for example. However, at the same time, it's fun to be involved in these professional...

20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...that of the gene of interest, or expressed from the same transcript as the gene of interest, but after an...