...interacts with the minor groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013)...

... (Cao et al., 2024). The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-...

...genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the late genes...

...initiation proteins. These iterons vary in their sequence and number and can alter the efficiency of replication...

.... Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive equipment...

... miRNA target sites, and recombination target sequences, denoted by triangles] facilitate tissue or cell...AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https:/...N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-specific enhancer...

...computer program for the construction of polylinker sequences of molecular vectors". Proceedings of the Belarusian...

...2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize to a different area of...

...interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of this portion ...considered a double-edged sword with unintended consequences. In this blog, we’ll take a deep dive into integrase-deficient..., & Ciuffi, A. (2012). Viral Integration and Consequences on Host Gene Expression. Viruses: Essential ...

...analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure...

...The Rinehart lab synthesized 110,139 unique DNA sequences corresponding to every previously observed instance...

...ll, of course, mention Addgene as a source of sequenced and quality controlled plasmids and viral vectors...

...Denature the samples to their primary amino acid sequence by boiling in the presence of a reducing agent...

...gene is very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational...

...expression vectors for insertion of custom gRNA target sequences....Cloning enzyme used for insertion of your gRNA sequence, such as BsbI or BsaI Cas9 (or other CRISPR) system...

...are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016; Hudry ...

...antibody’s identity to be easily confirmed via sequencing, and enables the variable region and constant...the DNA is released from the complex and can be sequenced and amplified for analysis. Overview of application...

...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence B PERB11.2...FAM3C family with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member...transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence b DKFZp686J10223...ERR3, FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76...Cas-Br-M (murine) ecotropic retroviral transforming sequence c CBL-3, CBL-SL, RNF57 CD247 CD247 molecule CD3... retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF from the labs...

...and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. In some...

...cell therapeutics because they can help start a sequence of immune reactions that aid in the fight against...