We narrowed to 526 results for: sequence

...2) non-targeting guides (red), which target no sequence in the genome, and are commonly used as negative...

.... Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive equipment...

... miRNA target sites, and recombination target sequences, denoted by triangles] facilitate tissue or cell...AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https:/...N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-specific enhancer...

...interactions between proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression...

...plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell type-specific...

...computer program for the construction of polylinker sequences of molecular vectors". Proceedings of the Belarusian...

...2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize to a different area of...

...analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure...

...interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of this portion ...considered a double-edged sword with unintended consequences. In this blog, we’ll take a deep dive into integrase-deficient..., & Ciuffi, A. (2012). Viral Integration and Consequences on Host Gene Expression. Viruses: Essential ...

...Denature the samples to their primary amino acid sequence by boiling in the presence of a reducing agent...

...Recipient cells express DsRed within a lox-STOP-lox sequence, followed by the EGFP, and the TEV protease. Once...

...ll, of course, mention Addgene as a source of sequenced and quality controlled plasmids and viral vectors...

...gene is very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational...

...are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016; Hudry ...

...cell therapeutics because they can help start a sequence of immune reactions that aid in the fight against...

...therefore necessitates careful design of RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and...

...pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928): p. 797-801. PubMed ...

... In addition, rAAV2-retro’s altered amino acid sequence might facilitate interaction with uptake or intracellular...

...tail sequences (κ pA), a CMV promoter for heavy chain expression, and an ER signal/leader sequence (L) ...light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain across...

...Antibiotics DNA Sequencing Sanger Sequencing Next-generation Sequencing Long-read Sequencing Reference Tables...of interest. Sequencing technologies like Sanger sequencing and next-generation sequencing (NGS) take advantage...Sanger sequencing, sometimes referred to as chain-termination sequencing or dideoxy sequencing (Figure...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid...vectors must contain a promoter sequence, a transcription terminator sequence, and the inserted gene. An expression... of ampicillin. DNA Sequencing for Plasmid Verification Scientists often sequence DNA to identify the ...know some of its sequence to design a effective primer. To learn more about the sequencing technologies Addgene...