We narrowed to 538 results for: sequence

1. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   ...processes including RNA movement by tagging RNA sequences with an aptamer and RNA transcription by placing...

2. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   ...initiation proteins. These iterons vary in their sequence and number and can alter the efficiency of replication...

3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...2) non-targeting guides (red), which target no sequence in the genome, and are commonly used as negative...

4. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   ...interacts with the minor groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013)...

5. An Introduction to Adenovirus

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   ...genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the late genes...

6. How to Negotiate a Successful Lab Start Up Budget

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   Blog Post

   .... Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive equipment...

7. Viral Vectors 101: Systemic Capsids

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   ... miRNA target sites, and recombination target sequences, denoted by triangles] facilitate tissue or cell...AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https:/...N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-specific enhancer...

8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of this portion ...considered a double-edged sword with unintended consequences. In this blog, we’ll take a deep dive into integrase-deficient..., & Ciuffi, A. (2012). Viral Integration and Consequences on Host Gene Expression. Viruses: Essential ...

9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize to a different area of...

10. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ...analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure...

11. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ...computer program for the construction of polylinker sequences of molecular vectors". Proceedings of the Belarusian...

12. COVID-19 Resources

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    Collection

    ... Plasmids Many plasmids containing SARS-CoV-2 sequences are now available and more are currently undergoing...

13. CRISPR Plasmids - Empty gRNA Vectors

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    ...expression vectors for insertion of custom gRNA target sequences....Cloning enzyme used for insertion of your gRNA sequence, such as BsbI or BsaI Cas9 (or other CRISPR) system...

14. Rinehart Lab Phosphoprotein Reagents

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    ...The Rinehart lab synthesized 110,139 unique DNA sequences corresponding to every previously observed instance...

15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post

    ...ll, of course, mention Addgene as a source of sequenced and quality controlled plasmids and viral vectors...

16. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ...Denature the samples to their primary amino acid sequence by boiling in the presence of a reducing agent...

17. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...gene is very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational...

18. Antibody Guide

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    ...antibody’s identity to be easily confirmed via sequencing, and enables the variable region and constant...the DNA is released from the complex and can be sequenced and amplified for analysis. Overview of application...

19. Immunology Research Plasmids and Resources

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    ...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence B PERB11.2...FAM3C family with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member...transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence b DKFZp686J10223...ERR3, FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76...Cas-Br-M (murine) ecotropic retroviral transforming sequence c CBL-3, CBL-SL, RNF57 CD247 CD247 molecule CD3... retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF from the labs...

20. Brain Armamentarium

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    ...-minBG-iCre-WPRE-BGHpA AiE2116m is an enhancer sequence, designed to drive AAV-mediated transgene expression...