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We narrowed to 846 results for: tat

Showing: 481 - 500 of 846 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...useful for neuronal tracing, it has two practical limitations. First, these high levels of viral replication...an enveloped virus. In general, viral envelopes dictate infection of target cells through their interactions...evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb. Proc Natl Acad Sci U ...

  3. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...which is then implanted into a female animal for gestation. Nucleases that induce double-stranded DNA breaks...which is then implanted into a female mouse for gestation. All three can introduce multiple base pair deletions...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...review analyzing and explaining the antibody crisis states that up to 50% of all commercially available antibodies... technology, it is important to show through qualitative data that they are a long-term solution to reproducibility... above) and literature suggest, providing an expectation and comparison on the antibody signal’s location...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...creativity and expertise of the group and yet never hesitated to reach out to the greater research community...portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus...and the School of Molecular Sciences at Arizona State University. His group carries out research in synthetic...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... the NIH, started off with the first keynote presentation. He talked about the impact of the Human Genome...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...species for genome engineering. The final keynote presentation was by Nobel Laureate Thomas Steitz of Yale....

  7. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...difficult person: 1) Understand your supervisor’s expectations Every supervisor has their own management style...frequently. The easiest way to meet your supervisor’s expectations is to ask upfront: “How frequently would you... details of your work. Describe the problem by stating the facts and explain how it is interfering with...

  8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...genetically modified organisms, and GMOs have a bad reputation. The public is can be fearful of this technology...ended up applying to a business accelerator freely stating that we had an idea, knew how to build it, but ...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The most common production...immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (Aurnhammer et al., 2012), droplet...what Addgene uses, check out our ddPCR for AAV quantitation blog post.  We generally do not recommend using...

  10. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids requested, categorized...total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that centers around the impact...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... of many cellular mechanisms, there are some limitations to working with FPs. Overexpression of fluorescently...of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One way to...However, CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput...

  12. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... clearly As a manager you need to clearly state expectations and goals. Yours and your team’s success ... schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...proteins that can be easily modified in order to facilitate its entry into the target cells (Beatty et al...tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports paper introducing... (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow 81...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...this process accounts for a percentage of the mutations seen with TALENs and CRISPR. MMEJ is active during...using much smaller regions of homology, which facilitates easier cloning. An overview of PITCh. ...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...

  15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...the new protocols.io beta platform for sharing, annotating, and storing life science protocols. Since we...simultaneously allowing scientists to actively comment, annotate, and edit protocols. After my introduction to ...lab-use tablet? The software also makes it easy to annotate protocols, make them public for others to use,...

  16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...protein synthesis. The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it....when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA during selection if it...good choice for experiments in which off-target mutations are undesirable. Pros: Works across many species...

  17. An Introduction to Adenovirus

    Type
    Blog Post
    ...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some of the ...doi.org/10.1038/s41586-023-05949-1. PMID: 36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...nothing’s ever perfect, neither is AAV. Its main limitation is the small size of its DNA cargo - 4.8 kb at...strategies are being optimized to overcome this limitation, with limited success so far. Luckily for patients...an ever-growing number of clinical trials for devastating genetic diseases such as hemophilia, muscular...

  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...attend? What is the expectation on level of critique and detail? Who is/are the facilitator(s) of the journal...papers could be more important.  Any slides, presentations, recordings, and other materials should be made...
Showing: 481 - 500 of 846 results