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...and many of the existing GCE tools are not very robust. When using GCE, each component of the experiment...turning GCE tools developed in the literature into robust and universal techniques that researchers can easily...

...tendency to go back and forth on email rather than just picking up the phone and having a discussion. By...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...

...CRISPR diversity is impressive, and researchers are just beginning to tap the wealth of CRISPR possibilities...respectively. Cas13d CRISPR array processing is robust, with CasRx and either an unprocessed or processed...

...presentation by Professor Guido Kroemer from the Institut Gustave Roussy. Professor Kroemer discussed the effects...26343581. PubMed Central PMCID: PMC4597191. 5. Eyquem, Justin, et al. "Targeting a CAR to the TRAC locus with...

...Blue-white selection is a widely used method to do just that! (To read about other screening strategies,...are a few common vectors. Blue-white screening is just that – a screening process. It does not select only...

... and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9 Synergistic...reports, “Here we have shown that the SAM system is robust, specific, and can facilitate genome-scale gain-of-function...

... Gordon Conferences). You don’t want to be so exhausted that you can’t get a lot from the presentations... plan to meet up at the next conference. Once is just a meeting, but having lunch twice turns a stranger...

...genetic modification (GM) and synthetic biology. The mistrust and anger directed at GM crops has burdened this...imagine any use for GM technology other than to sustain those techniques. We feel strongly that the scientific...

...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells...

...thanks for looking me up on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are...networking--”If it feels creepy, don’t do it.” It’s just not a useful way to build or use a network. Total...

...targeting Cas enzymes Move over Cas9, CRISPR isn’t just for DNA anymore! Cas enzymes have been isolated ...traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally. Cas13 Cas13 is an RNA-guided...

...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...there are many other amazing affinity reagents we just didn't have the space to give proper credit to. ...

...molecules. With the direct approach, primary antibodies must be either sourced already conjugated or conjugated...different signaling molecules. Many labs find that a trusted stock of secondary antibodies saves time and money...

...Exclusively Activated by Designer Drugs (DREADDs) (Armbruster et al., 2007).  We initially used a construct...disorders.   References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007...

...were covered in the workshop. These workshops illustrate the open and modular nature of the workshop content...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...

...interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name a few. But what if you want to study RNA-protein...serum iron levels seen in HHCS patients. These are just three examples of how RaPID can be used to study...

...DNA sequence and RNA expression - but what if you just want to change protein levels? Until recently, no... no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away - an antibody-based...

...a scientist. I pretty quickly figured out that I just don’t have the patience to be a scientist,” says....  Another difficulty is that some articles are just harder to write. “There’s something about being ...

...and, like the game of telephone, often the signal must be transmitted by intermediate messengers. Optical...Western blot. Phosphorylation of MEK1/2 peaks at just 5 minutes post-Raf1 inactivation, and then decreases...

...time of writing, Brigham And Women's in Boston also just announced that they were reversing their plans to...to pursue academic work, and to enhance the sustainability of the scientific enterprise by reducing the...