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We narrowed to 938 results for: Kin

Showing: 251 - 260 of 938 results

  1. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...to the meeting, and its history of code making and code breaking is wonderfully analogous to genetic circuits...our baffled flight attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited...as an undergraduate, I never fooled myself into thinking I was an engineer. The talks we heard Monday night...discussed in the book). These synthetic biologists are taking the "genetic code" to a new level, changing it ...years. I kept busy for the whole poster session, talking to people from every conceivable background at ... Eric J. Perkins...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...Joanne's Reddit AMA  Few of us spend much time thinking about conserving resources in the labs. Disposable...Founder of Seeding Labs, tells the story about working as a student in a rice lab in the Ivory Coast where...very environmentally conscious friends who had me thinking about energy conservation even before it was a...accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, ...founder, Melina Fan, recently shared our protocol for making competent cells to save time and money. My other... portion of the gel.  What a waste?  I started looking for smaller and smaller gel apparatus until I settled... hilarious.  Birth of the baby gel box Finally, taking conservation to new levels, I was bothered by the...

  3. The Strength of Story Telling

    Type
    Blog Post
    ...I’m working with a talented journalist to shine light on living as a scientist in Beijing, talking to ...to people about taking beautiful photographs of their studies, reporting on amazing women in science, or... or asking someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal...We build narratives around the self, too — when talking to friends or dates or colleagues we tell tales... a solid point to kick off at. Whatever you’re working on, start wherever it makes sense to start and ... overnight. The only way to improve is to keep working on telling a story again and again and again until...

  4. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...different kinds of grants that you can apply for. If you are in a lab, your PI is likely working on a number..., we posted these 25 great tips for scientists working at home, and you will still find this useful. Now...been doing this for almost 6 months, you may be looking for more ways to make sure you are moving forward...Addgene have gone virtual!   2. Practice public speaking For almost any science career, there will be a...good progress on both building relationships and thinking about future career options. 5. Write a grant ...Science If you have a method that you've been working on, let us know in the comments below! Additional...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    .... Both have significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for...larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of large populations...generation and stable cell line creation (Figure 2). Looking for more? Explore our empty and EGFP retroviral...endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to insert the payload sequence ...Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein...Cas9 nickase to improve target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety... on other proximity ligation tools with better kinetics and less background labeling. By fusing miniTurboID...

  6. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches...transgenic cell lines. The traditional method of making stable cell lines is to transfect, select, and ...negative impacts on fitness. One consideration when making a transgenic cell line with the piggyBac™ system...However, several challenges still remain, such as making gene edits completely seamless (i.e., so no other...piggyBac™ transposase (PBx), which contains mutations making it competent for excision, but defective for integration...containing a negative selection marker such as thymidine kinase, a researcher may subsequently kill off any cells...target sequence. This allows researchers to avoid making unnecessary base pair changes as would be the case...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...everything click in my head and left me thinking: I wish I had this kind of experience earlier! After all,...biology in high school, it wasn’t until I started working in my first molecular biology lab in college that...more and more molecular biology-based activities making their way into high school classrooms, like gel... on freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free extracts...the protein in a cell-free manner. This is like taking an engine out of the car – you can still use the...cost-effective, as each one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...useful to anyone working in a lab.   Participating in a workshop at lab central We’re working on a project...of experimental services and expertise without breaking their bank accounts. When I heard about how LabCentral...partner for the day was Julie from Novartis who was working with cell culture and who was extremely helpful... now know what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with...new without spending a lot of time developing and making space for the techniques ourselves. As you probably...should give similar workshops a try if you’re ever looking to try out a new technique or reagent! Racheal...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...projects range from hacking probiotics to filming educational lab videos, while some are looking to develop new...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the... gene drive paper was very popular. People kept asking if we were teaching classes at the lab, so in December...That’s right, we have non-traditional scientists working in our laboratory! Don’t worry though, we make ...transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse range of...

  10. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Addgene, who will be taking over the distribution to expand the network of scientists working on this even more...throw a therapeutic monkeywrench into the virus’ hijacking of human cells. Even better, more than half the...the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since...interest, because it means more scientists will be working on this problem more quickly. Figure 2: Getting...headache receiving constant email requests and tracking their fulfillment manually in a shared spreadsheet...interested in facilitating collaborative science, and making it accessible to all people, not just scientists...
Showing: 251 - 260 of 938 results