We narrowed to 643 results for: ATO

1. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

   ...Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer...

2. Making CRISPR Plasmids Using Fragmid

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   ...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

3. Viral Vectors 101: Chemogenetics

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   Blog Post

   ...PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal of creating...

4. Viral Vectors 101: Virus Safety

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   Blog Post

   ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-...

5. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

6. Hot Plasmids and Viral Preps - January 2021

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   ...AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5...

7. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical...

8. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   Blog Post

   ...editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently edited the fetal...

9. Engaging with science and society at pgEd

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   Blog Post

   ... in genetics research, their applications and regulatory policies; keeping tabs on the conversation about...

10. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

11. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...Destination vectors. These vectors can induce combinatorial constitutive or inducible gene overexpression...

12. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...

13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...

14. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...general consideration for behavioral testing in a laboratory, a high level of experimental control is required...

15. CRISPR in the Clinic

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    ... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...

16. 5 Tips for Troubleshooting Viral Transductions

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    ...sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has been shown to increase...

17. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...

18. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ...the work I agreed upon (just as I would in my laboratory work). Committee members have to work professionally...

19. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...trying to explore the links between genetics and anatomy, which are not yet fully understood. We attempt...

20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post

    ...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...