We narrowed to 1,015 results for: LEA

...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

...critical roles in nerve and muscle function, hormone release, and cell signaling throughout the body. Because...Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR). ...

...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

...scientists? Curiosity? Passion? A love of learning? No. Or at least not entirely. What truly unites us all...

...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...blog posts on base editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog...

...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb...

...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

...Symposium on Cancer, Inflammation and Immunity and learn more about “... recent findings in basic immunology... autophagy can be found here. Fun fact: I also learned that Professor Kroemer often functions as one of...

...separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al, 2010). An explanation...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

...ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base... as cell lineage studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using the ...

...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

...reprogramming, and beyond. For further details, please check out Wong et al 2016 and Wong et al 2015. ...PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA Read How ...

...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...easier. Advantages and other possibilities It’s clear that Cre-DOG and T-DOGGs open up many new possibilities...

... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...

...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For...PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. ...

...upstream of the transcription initiation sites of at least two genes with high expression and high cell-type...  Targeted expression was defined as meeting at least one of the following criteria:  More than 90% of...

...performed, optimized as needed, and cleaned up using a standard PCR cleanup kit to remove any template DNA ...

...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...

...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...

...ever like to write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode...accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust, when integrated...

...on the Addgene Blog Learn about techniques for visualizing protein turnover Learn about plasmids for in...

... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...

...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...

...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...