Addgene

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Cultivating Community Science at BosLab

Type

Blog Post

... BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located...
Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Type

Blog Post

...if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in...
Addgene Welcomes John Doench to the Advisory Board

Type

Blog Post

...during a lecture given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp...
Plasmids 101: Mammalian Vectors

Type

Blog Post

...Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd HeLa, NIH3T3, CHO, COS...
Addgene’s Viral Service - Why Virus? Why Now?

Type

Blog Post

...ice. The Business and Finance Teams continue to facilitate the formation of partnerships to expand the service...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

... means recombinant proteins can aggregate or precipitate out as insoluble granules. We overcame this problem...
Behind the scenes: Addgene’s new search engine and more

Type

Blog Post

...engine unique. Most importantly, with this new implementation, we laid the foundations for many improvements...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

...approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine which enzyme...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...
Viral Vectors 101: Viral Applications

Type

Blog Post

... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...by long terminal repeat (LTR) sequences, which facilitate integration of the transfer plasmid sequences...
Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

...). Figure from Andrews, et. al., Elife A limitation of hybridoma generated mAbs is that if the frozen...
Antibodies 101: Isotypes

Type

Blog Post

...that helps attack extracellular pathogens. Transportation of antibodies to places they can’t reach on...
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Type

Blog Post

...discovered in 1946 by Joshua Lederberg and Edward Tatum when they observed genetic recombination between...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...
Early Career Researcher Toolbox: Social Media for Scientists

Type

Blog Post

...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

...create chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural...
A Quick Guide to a Career in Software Product Management

Type

Blog Post

...catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss which front-...
How Addgene Creates Content

Type

Blog Post

... be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies ...
How to Make Friends and Meet People at a Scientific Conference

Type

Blog Post

...exhausted that you can’t get a lot from the presentations and programs. After the conference Follow ...
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Type

Blog Post

... to achieve success in this business. A good presentation can help win a business plan competition and...
CRISPRainbow and Genome Visualization

Type

Blog Post

...fluorescently labeled Cas9’s come with a number of limitations. Since color is specified by PAM sequence, each...
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

...actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...modeling. Its reversibility allows precise and quantitative analysis of epigenetic memory at nearly any ...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...
To Each HIS Own

Type

Blog Post

... H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel...
Plasmids 101: The protein expression toolbox

Type

Blog Post

... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...
All in a Twist: dsRNA

Type

Blog Post

...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Type

Blog Post

...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...
Antibodies 101: Polyclonal Antibodies

Type

Blog Post

...protein in low abundance or whose conformational state may change. If your protein might become slightly...

Showing: 751 - 780 of 836 results

Results per page:

Cultivating Community Science at BosLab

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Addgene Welcomes John Doench to the Advisory Board

Plasmids 101: Mammalian Vectors

Addgene’s Viral Service - Why Virus? Why Now?

Developing a cold tolerant E. coli using specialized chaperones

Behind the scenes: Addgene’s new search engine and more

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Viral Applications

Viral Vectors 101: Gamma-Retroviral Packaging Systems

Building and Validating Recombinant Antibodies: A Case Study

Antibodies 101: Isotypes

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Early Career Researcher Toolbox: Social Media for Scientists

GPCRs: How Do They Work and How Do We Study Them?

A Quick Guide to a Career in Software Product Management

How Addgene Creates Content

How to Make Friends and Meet People at a Scientific Conference

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

CRISPRainbow and Genome Visualization

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

To Each HIS Own

Plasmids 101: The protein expression toolbox

All in a Twist: dsRNA

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Antibodies 101: Polyclonal Antibodies

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