We narrowed to 1,100 results for: Ide

1. Expanded Access to Rabies Plasmids

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   Blog Post

   ...international institution. Requesters will need to provide a brief description of the applicable research ...

2. 8 Great Depositor Research Stories

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   ...their paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation...

3. DIY DNA Ladders from Penn State University

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   ...ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying Your Plasmid ...

4. Bringing Renewable Energy to Addgene

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   ...implemented at Addgene. The kitchen and office areas provide multiple streams for composting and recycling in...

5. Call for Guest Bloggers

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   ...Blog Possible guest topics Some topics you might consider are: An overview of software tools useful to ...

6. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

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   ...taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the article to...

7. CRISPR Cheat Sheet

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   ...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...

8. AAV Packaged on Request is Here!

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   ..., plus viral genomic sequencing to confirm the identity of the packaged DNA and serotype. That way, you...

9. Editor's Choice, October 2016

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   ... incredibly important information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased...

10. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

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    ..., and suitable for molecular cloning. Plus, we decided to include 10 plasmids that can be directly used...

11. 2018 #DeckTheLab Contest Winners

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    ...the North Pole to New York City. Check out their video below. Happy Holidays from the @SikoraLab! This...

12. Grad School Advice Part 1: Picking a Lab and a Project

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    ...approach doing rotations? 8:01 - 11:25 : How did you decide what lab to join? 11:25 - 11:50 : What is the number...

13. Editor's Choice, July 2016

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    ...contributed by our bloggers each and every month, we've decided to start an "Editor's Choice" series. Each month...

14. Grad School Advice Part 2: Building Community

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    ... hard about picking a good mentor, and begin considering career options early. Tune in for great advice...

15. Fueled by Coffee at #SfN14

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    ...astrocytes to functional neurons in vivo. And I’ve seen evidence of the infiltration of CRISPR-Cas tools into neuroscience...

16. How Do I Place an Addgene Order Using a PO Number?

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    ...order will be sent to the contact email addresses provided. How do I place an order using a PO number? Placing...

17. How Exercise Purges Stress and Keeps Depression at Bay

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    ...plenty more to explore. “It will be important to identify the key proteins and metabolites that are dynamically...

18. Addgene's Top Blog Posts from 2020

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    ...DNA? Here are Five DNA Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate...

19. Designer PUF Proteins for Any RNA Target

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    ...recommends using standard BsaI in place of the high fidelity version (despite what the original paper says)...

20. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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    ...mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids...

21. Let There Be LITE Plasmids

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    ...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

22. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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    ...microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...

23. Mapping the 4D nucleome with CRISPR/Cas9

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    ...available from Addgene!) Since the signal level provided by SunTag is so high, cells may be imaged under...

24. Cre-ating New Methods for Site-specific Recombination in Drosophila

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    ... Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance for the efficiency of...

25. Year of the Camelid: Antibody Style

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    ...as new sdAbs become easier to generate and more widely adopted. So thank you to the camelids who inspired...

26. Sleeping Beauty Awakens for Genome Engineering

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    ...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

27. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    ... ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He ...

28. Meet the New Addgene Board Members!

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    ...took a dividing yeast and did a genetic screen to identify the factors that regulate cell division. And that...

29. New and Upcoming Viral Vectors - Spring 2019

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    ...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

30. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ...research.     To see Tetbow in action, watch the video below depicting dendrite wiring of mitral cells....

31. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    ...seen the emergence of several SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective...

32. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ... as a seizure disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3...

33. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...antigen, pre-existing conditions, etc. that can vary widely between recipients.   In vitro prediction of drug...

34. Hot Plasmids - January 2023

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    ...stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains...

35. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    ...equilibrium with ambient air is restored. We have no evidence to suggest that the transient change in pH affects...

36. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...reporter that would fluoresce: FlipCherry. FlipCherry provides a proof of concept that the designed used in FlipGFP...

37. Networking Follow-up: I Met a Person. Now What?

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    ...week) so they can easily recall the encounter. As a side note, my guess is that people will not be exchanging...

38. Synthetic Photobiology: Optogenetics for E. coli

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    ...in optimizing TCSs. Over 75,000 TCSs have been identified in bacteria, and with similar optimization methods...

39. Antibodies 101: Isotypes

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    ... isotypes, such as the number and location of disulfide bonds, the number of constant domains, and the...

40. Building and Validating Recombinant Antibodies: A Case Study

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    ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...