...bioanthropology. Studying human health science made me happy, but it wasn’t long before I started becoming disillusioned...drives better after a few drinks. A family member approached me for advice on her baby’s health but adamantly...abstract where the real medical conditions had been swapped with made up illnesses. They were then asked to...

... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

...(Schrallhammer et al 2012). This made them an appealing target for bioengineering, but it wasn’t clear...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...

...flank the insert. You will need to know both the approximate size of the vector backbone as well as the predicted...you have loaded in each well will allow you to approximate the DNA mass of comparably intense samples of...the gel when the bromophenol blue dye line is approximately 75-80% of the way down the gel will ensure you...

...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...dream lab While none of us will feel completely happy at work all of the time, the goal is to spend as...the difference. Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments...

...infectivity. Next, the virus must assemble and have appropriate factors displayed on the outside of the particle... production, they can be helpful for certain applications and aid in ease of use. Our lentiviral guide...elements discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake...

...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this... this blog we will focus on those which can be applied in cell culture and animal models primarily. Fun...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

... exon skipping. Previous CRISPR exon-skipping approaches used two guide RNAs to remove a given exon from...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d...sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools available...

...guidance and structure. I quickly learned that my approach needed to adapt based on individual needs. For... and disengagement. By tailoring my leadership approach to each person, I was able to create a more productive...tension between two team members with differing approaches—one detail-focused and the other big-picture ...

...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...intermediate won’t just have nicks: it may have gaps or overlapping regions of single-stranded DNA. But as long as...of multicomponent assembly, as fragments with overlapping sequence homology can be assembled in a unique...

...can firmly gain a handle on understanding and appreciating their biology. The importance of psychrophilic... the empty vector. And thus was born ELSA - our happy lil’ cold-tolerant E. coli! Additionally, we’re ...re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a...

... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

... and remove a pathogen. Different isotypes also appear at different stages of an immune response. There... isotype of an antibody so you can select an appropriate isotype control. An isotype control has the same... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

...interview or whether your receive the next grant you apply for. After all, the only value your work will have... slide, think about whether the same principle applies to other slides in your deck.  If someone asks ...stay tuned to our website and social channels to apply for our future events. In addition to this annual...

...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...teams around the world who combine engineering approaches and scientific know-how to design/build/test ...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...

...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system...multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate ...

...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation...but there are several hazards to navigate when mapping out a new project. We’ve shared some of the concerns...

... I will describe some of the many ways you can approach finding someone to give you advice and guidance...see someone with a great professional presence, approach them for advice on what makes them that way. Is...organization has a mentoring program, consider tapping into it and helping make it work by being an educated...

...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...rates for AAVs are generally high enough for this approach to work, although it’s less effective in vivo ... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of...method is free for you, and many labs are quite happy to share, labs that get high volumes of plasmid ...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...