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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 641 results for: CAR

Showing: 501 - 520 of 641 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...

  2. General Transfection

    Type
    Protocol
    ... 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations...DNA tube. Incubate the mixture 15–20 min at RT. Carefully transfer the transfection mix to your HEK293T ...cells. Add the transfection mix dropwise, being careful not to dislodge the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 15 ...

  3. Using a Light Microscope Protocol

    Type
    Protocol
    ...move your microscope around the lab, be sure to carry it with two hands - one hand supporting the base...base and the other holding the arm - or use a cart. Revolve the nosepiece so that the lowest power objective...objective. Take your eyes away from the eyepiece and carefully revolve the nosepiece so that the next objective...will notice that the objectives get larger. Be careful to avoid hitting your sample with the objective...laboratory instrument, you should be sure to take care of your microscope. Bumps, scratches, and dust can...

  13. To Each HIS Own

    Type
    Blog Post
    ...proteins will refold once denatured, however, so take care when using this method.      Fig 2:  A, ...

  19. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...the second stop and carefully raise the pipette straight out of the buffer. Carefully load your samples ...occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling can occur. Pro-Tip It...not added EtBr to the gel in the first place. Carefully load a molecular weight ladder into the first ...the electrodes from the power source, and then carefully remove the gel from the gel box. Optional: If ...
Showing: 501 - 520 of 641 results