We narrowed to 637 results for: CAR
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TypeBlog Post...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...
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Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley Listen to the anti-CRISPR podcast segment... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...different plasmid backbones that varied in size or carried different genes. In these cases, gene expression... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction... -
To Each HIS Own
TypeBlog Post...proteins will refold once denatured, however, so take care when using this method. Fig 2: A, ... -
Plasmids 101: The protein expression toolbox
TypeBlog Post... be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for integration at a... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...Epitope availability References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...proteins of interest - the secondary antibody needs careful consideration before selection. If all the primary... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...Sergi, Jacob J. Hughey, Bryce T. Bajar, Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements... -
Developing Lab Management Software for Biology
TypeBlog Post...mushy, gooey contents of said containers, think carefully about how you want your software to treat those... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...distribute through our deposit process, and then we carefully track samples through our lab using barcoded tubes... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post.... PubMed Central PMCID: PMC4139937. 3. Anders, Carolin, et al. "Structural basis of PAM-dependent target... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...like papers that can end with some sort of model (cartoons are appreciated) to visually sum up all of the... -
General Transfection
TypeProtocol... 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations...DNA tube. Incubate the mixture 15–20 min at RT. Carefully transfer the transfection mix to your HEK293T ...cells. Add the transfection mix dropwise, being careful not to dislodge the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 15 ... -
Using a Light Microscope Protocol
TypeProtocol...move your microscope around the lab, be sure to carry it with two hands - one hand supporting the base...base and the other holding the arm - or use a cart. Revolve the nosepiece so that the lowest power objective...objective. Take your eyes away from the eyepiece and carefully revolve the nosepiece so that the next objective...will notice that the objectives get larger. Be careful to avoid hitting your sample with the objective...laboratory instrument, you should be sure to take care of your microscope. Bumps, scratches, and dust can... -
A Look at Addgene's QC Process
TypeBlog Post...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources Additional... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...generally less common and will stand out; however, be careful not to hang your poster too low. If it is pinned...