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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 644 results for: CAR

Showing: 501 - 520 of 644 results

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...

  5. General Transfection

    Type
    Protocol
    ... 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations...DNA tube. Incubate the mixture 15–20 min at RT. Carefully transfer the transfection mix to your HEK293T ...cells. Add the transfection mix dropwise, being careful not to dislodge the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 15 ...

  8. To Each HIS Own

    Type
    Blog Post
    ...proteins will refold once denatured, however, so take care when using this method.      Fig 2:  A, ...
Showing: 501 - 520 of 644 results