We narrowed to 770 results for: Dos;
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TypeBlog Post...much international attention. Their small numbers do not, however, make these patients suffer any less...
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SunTag and Fluorescent Imaging
TypeBlog Post...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...arms so that they can hold and hug items closer and do so more animatedly. To communicate that sense of ... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... and GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...for change in their opportunities. Scientists must do science to be happy so the beginnings of solutions... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...mindful of the lab materials I was using. I started to do little things to save reagents and money. This was... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post... lentiviral CRISPR library. “This enables you to do customized genetic modification on a scale that was... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post.... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it? Test the serotype and promoter The first... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that... -
The Strength of Story Telling
TypeBlog Post... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide... -
Antibodies 101: Affinity Tags
TypeBlog Post...where affinity tags come in – they are peptides which do not naturally exist in most expression systems that... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...both bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools...