We narrowed to 821 results for: EED

... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...

...ABE8 (Gaudelli et al., 2020). The generated ABE8s needed to produce 3 concurrent base edits to withstand...editing single genes is very useful, sometimes, you need to modify multiple genes to generate a cellular ...

...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

... you can show off your work at a conference, you need to first make your poster. While a poster generally... to verbally communicate your work, so you don’t need every little detail on the board.  Poster layout...

...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...

...to knock in corrective genes. For this you would need to provide a DNA template to repair the double strand... homology directed repair and you would probably need to use a two viral vectors system: one vector for...

...tag reduces the number of CAPTURE components that need to be delivered to cells from three to two. Lentiviral... remaining CAPTURE 2.0 components eliminates the need to create stable cell lines and allows CAPTURE 2.0...

... reagents in three major steps - all without the need to find a llama farm! To find an optimally binding... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

...system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

...mammalian cells, or in purified form, and didn’t need any added cofactors.      Figure 2: Fluorescence... and often, including works-in-progress still in need of further study and development like MagLOV. The...

...protein you are trying to express How much protein you need   Your plans for downstream applications In this...than bacterial cells and growing conditions often need to be optimized. Yeast cells are also known for ...

... xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there is an ...Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter PAM sequences...

...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...

... pose the greatest threat, cancerous cells still need to successfully infiltrate the circulatory or lymph...include several other important pathways. We invite feedback and contributions from all members of the research...

...arm repair template (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination...endogenous genes in human cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene...