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We narrowed to 830 results for: EED

Showing: 501 - 520 of 830 results

  4. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...

  14. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

  16. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

  17. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

  18. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

  19. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...
Showing: 501 - 520 of 830 results