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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 825 results for: EED

Showing: 501 - 520 of 825 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...mammalian cells, or in purified form, and didn’t need any added cofactors.      Figure 2: Fluorescence... and often, including works-in-progress still in need of further study and development like MagLOV. The...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there is an ...Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter PAM sequences...

  9. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...protein you are trying to express How much protein you need   Your plans for downstream applications In this...than bacterial cells and growing conditions often need to be optimized. Yeast cells are also known for ...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...arm repair template (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination...endogenous genes in human cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene...

  13. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...CHRM4 gene. When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing...interrogating the VGS data for small predetermined seed sequences unique to the capsid plasmid. Our recombination...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48 clones from... your colonies are well separated. For this, you need to have some idea of the rate of growth of your ...

  16. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...all the time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of... example: Don't call me a dropout: Why science needs more people to quit the lab. Resources on the Addgene...

  18. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...pages contain all the detailed information you'll need to use the plasmid in your experiments: Backbone... plasmids).  We also like to store plasmids that need extra stability, such as some of our viral vectors...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...process has steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our...suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification steps ...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission wavelength range, there are other traits that need to be considered when choosing an FP: Unique categories...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...
Showing: 501 - 520 of 825 results