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  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

  8. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

  11. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...

  13. Plasmids 101: Protein Expression

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    Blog Post
    ...protein you are trying to express How much protein you need   Your plans for downstream applications In this...than bacterial cells and growing conditions often need to be optimized. Yeast cells are also known for ...

  14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there is an ...Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter PAM sequences...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...CHRM4 gene. When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing...interrogating the VGS data for small predetermined seed sequences unique to the capsid plasmid. Our recombination...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48 clones from... your colonies are well separated. For this, you need to have some idea of the rate of growth of your ...

  19. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...mammalian cells, or in purified form, and didn’t need any added cofactors.      Figure 2: Fluorescence... and often, including works-in-progress still in need of further study and development like MagLOV. The...
Showing: 501 - 520 of 816 results