We narrowed to 629 results for: EMB

1. Advice for Scientists Starting a Lab

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   Blog Post

   ...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...

2. Hot Plasmids - February 2022

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   Blog Post

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

3. Sweating the Small Stuff: Details in the Lab

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   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

4. Antibodies 101: Isotypes

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   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

6. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

7. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

9. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

10. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

11. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...

12. Viral Vectors 101: Viral Applications

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    Blog Post

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

13. Improved Plasmid Maps Powered by SnapGene

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    Blog Post

    ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

14. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

15. Multiplex Genome Editing with CRISPR-Cpf1

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    ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

16. Screening for Successful Genome Editing with Digital PCR

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    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

17. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

18. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

19. Summer SciComm: Preprints

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    Blog Post

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

20. Antibodies 101: Affinity Reagents

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    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...