Advice for Scientists Starting a Lab
Type
Blog Post
...scientists to their labs.
Further Reading & Resources
EMBO Lab Leadership Courses
HHMI Resources for Early ...
We narrowed to 629 results for: EMB
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Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...specific locus to create a knock-out in human embryonic stem cells. Find GEmCherry2 reporter plasmids... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post... in addition to testing their performance (2). Remember that although digital PCR is a great screening... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are... -
Hot Plasmids - February 2022
TypeBlog Post... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...side to your right as you use them can help you remember what's been added and what hasn't. Developing ... -
Antibodies 101: Isotypes
TypeBlog Post...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ... -
Viral Vectors 101: Viral Applications
TypeBlog Post...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post... works in Dale’s lab. Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the... -
Addgene: The First Twenty Years
TypeBlog Post...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...