We narrowed to 625 results for: Emb;

1. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

2. Antibodies 101: Isotypes

   Type

   Blog Post

   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

3. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

4. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

5. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

6. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

7. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

10. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

11. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

12. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

13. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

15. Summer SciComm: Preprints

    Type

    Blog Post

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

16. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

17. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...