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We narrowed to 527 results for: HA tag

Showing: 501 - 520 of 527 results

  1. RNA Extraction Without A Kit

    Type
    Blog Post
    ...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and less...

  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl substitutions...

  5. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...assistance in the grant application process, she has faith in you! Additionally, keep in mind that universities...of current methodologies. This section sets the stage for why your funded proposal will address these ...

  6. Engaging with science and society at pgEd

    Type
    Blog Post
    ...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...who are interested in such careers should take advantage of their academic environment and attend seminars...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...site-specific recombination. The FLEx switch takes advantage of the orientation specificity of the site-specific...

  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors, optogenetic...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...

  9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...about ELN costs and limited budgets, “a large percentage of survey respondents indicated that cost was...effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger, Tea Pavlek...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over in videos...: If you’ve ever watched any behind the scenes footage in the last several years, you can usually see ...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...fluorescent molecules, including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based...

  12. 6 Tips for Grant Writing

    Type
    Blog Post
    ...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ... Strategize, choose calls that suit your career stage / research area, and focus on producing a good quality...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...know this?” Over the past fifteen years, the CBM has developed a student-centered SMART Team modeling ... of proteins using 3D printing technology. The tagline for our company is “…where molecules become real...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6...

  15. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...help each other by sharing their data. So take advantage of it and get your data - negative AND positive...

  16. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...There are many moving parts to our software, which has grown with the company. It would be easy to assign...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will happen...Growth of new livers in culture could resolve this shortage." What is the knowledge gap you plan to address...

  18. How to Be an Excellent Trainee

    Type
    Blog Post
    ...the bench waiting for you, prep for research work has to be done by the researchers themselves.  Be ...rotation students, joining a new lab at any career stage, learning a new technique while visiting a lab, ...

  20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially...
Showing: 501 - 520 of 527 results