We narrowed to 520 results for: HA tag
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TypeBlog Post...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors, optogenetic...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...
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Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...about ELN costs and limited budgets, “a large percentage of survey respondents indicated that cost was...effort and give the lab a serious competitive advantage. Many thanks to our guest blogger, Tea Pavlek... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over in videos...: If you’ve ever watched any behind the scenes footage in the last several years, you can usually see ... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...fluorescent molecules, including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few. Find Many FRET-Based... -
6 Tips for Grant Writing
TypeBlog Post...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ... Strategize, choose calls that suit your career stage / research area, and focus on producing a good quality... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...know this?” Over the past fifteen years, the CBM has developed a student-centered SMART Team modeling ... of proteins using 3D printing technology. The tagline for our company is “…where molecules become real... -
AAVs in Retinal Gene Therapy
TypeBlog Post...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...help each other by sharing their data. So take advantage of it and get your data - negative AND positive... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will happen...Growth of new livers in culture could resolve this shortage." What is the knowledge gap you plan to address... -
How to Be an Excellent Trainee
TypeBlog Post...the bench waiting for you, prep for research work has to be done by the researchers themselves. Be ...rotation students, joining a new lab at any career stage, learning a new technique while visiting a lab, ... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...There are many moving parts to our software, which has grown with the company. It would be easy to assign...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...two flanking repeats. In cells where recombination has not occured, methanol induces the production of the...that retain the ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...genome-wide screens. Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at room temperature... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...introduction of an intended mutation while taking advantage of the improved efficiency provided by blocking... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...sequence and other regions of the genome. The main advantage of NGS is that it allows you to simultaneously... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...itself, like laser type and power, acquisition voltage, the concentration of the fluorophore, and whether...bd-spectrum-viewer. But what is the real-life advantage of a brighter fluorophore? Strong brightness makes... -
Finding Your Science Policy Path
TypeBlog Post...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items: A simple place to...an account and start engaging with the #scipol hashtag. Often there will be posts about virtual meet ups... -
Trimmer Lab NeuroMab Collection
TypeCollection.../9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [N77...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful for... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin... Mouse Mouse IgG2a 177526 Anti-Synaptotagmin-7 [N275/14R] Synaptotagmin-7 Mouse Mouse IgG2a 177527 Anti-Lgi1... Human Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK...