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We narrowed to 989 results for: IGH@;

Showing: 501 - 520 of 989 results

  1. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... together on a tight budget. To help popularize our kit resources,  we’re highlighting just a few of ...covering a wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections...Morgan’s blog post on the PUF Assembly Kit for some insights on its potential from its creators. We hope you...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ...amount of high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput...method called pyrosequencing. The drive for faster, higher throughput and less expensive sequencing fuels ...commercially available although they are reported to have high error rates (10). It is projected that nanopore-...and research progress. These will bring faster, higher throughput, and potentially more accurate techniques...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...untreated individuals. Symptoms of AIDS include rapid weight loss and an increased risk of infection, including...models, and it’s difficult to tell if one approach might be more successful. “Shock and kill” has the advantage...expression of Cas9/gRNAs, which could result in higher rates of off-target cleavage. In both cases, translating...each of these gRNAs will need to be validated for high on-target binding and low off-target binding. It...stable expression of Cas9/gRNA. Viral production is higher in this model than in latent HIV-1 infection, which...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...the perfect empty backbone, made sure to add the right tags to your gene, and may have even put a fluorescent...are marketed for a specific purpose: fast growth, high-throughput cloning, routine cloning, cloning unstable...  Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression...synthesis Allows uptake of large plasmids hee   High electroporation efficiency supE44 (glnV44)   Suppression...+)  XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of large plasmids...

  5. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some... Andrew Willoughby introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE...

  6. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...community like Watertown to bulk purchase energy from a higher percentage of renewable sources. This program offers...sources and we have chosen to participate at the highest level offered. As a result, we will be drawing ... our electricity from renewable energy projects right here in New England- the majority of which are solar...temperatures, biosafety cabinet ventilation, and lab lighting/HVAC requires a significant energy expenditure...

  7. Deck the Lab 2022!

    Type
    Blog Post
    ...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....in our building. Unfortunately, snow is thawing right now, so today noone could see my (not so) little...                     A post shared by 草方 (@gracie_fang) That’s right, researchers of the world. Cap your Eppendorf tubes...Instagram! Happy decorating, all, and to all a good light!    🤩 our lab in Bayliss Building @SMS_UWA is getting...

  8. New Viral Vectors - March 2024

    Type
    Blog Post
    ...vector pAAV-CAG-RdLight1 AAV9 Biosensor Tian New viral vector pAAV-hSynapsin1-RdLight1 AAV9 Biosensor...lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (AAV9), which contains a biosensor that...Biosensor Tian New viral vector pAAV_hSynapsin_psychLight2 AAV9 Biosensor Tian New viral vector pAAV.hSynapsin.SF-iGluSnFR.A184S...

  9. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...that infectious wild-type viruses were produced in high-titer retroviral stocks generated by the transfer...In this kind of approach, a population of cells might express only the tTA or your transgene resulting...developed, but these vectors are quite large and you might get lower viral titer when using them.   Regarding.... If you are transducing non-adherent cells you might need to centrifuge your cells with the virus in ...Trouble Shooting Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral Vector...

  10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination ...induces a noticeable color change. This method has high sensitivity but low specificity (Degeling, 2012)...required in this process because result interpretation might be difficult. The low density of mycoplasmas, contamination...media as much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid...exposure of the cell culture to air and make sure to tighten the flask cap before placing them in the incubators...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...decades of my life following a very straightforward conveyor belt from high school to college to graduate school...Veterans Health Administration, I could never get one straight answer about what science policy even is! In this... “little p” policy.  “Big P” policy is what you might typically think of. It occurs in a government setting... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

  12. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...and four chaperones from Denis-Yarmouth Regional High School visited our labs in Cambridge, MA to learn... Outreach Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European...Website Learn more about Dennis-Yarmouth Regional High School Resources at Addgene  Read more Inside ...

  13. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...working with AAV or lentivirus in your research, you might want to use one of our virus backgrounds. Addgene...backgrounds for you as well! If you use FRET, you might be interested in our fluorescent protein background....           Did you get the joke? If not, you might want to check out Addgene’s Fluorescent Proteins...background.     We hope these science backgrounds will brighten up your virtual meetings!  ...

  14. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...It’s well known that exercise can help fight stress and depression, and now researchers have reported...including unpredictable loud noises and flashing lights - that sent average, control mice into depression...know about you, but I think I could use a good run right about now. And, if you are looking to get your hands...for studies in your own lab, you’ve come to the right place. Get started with PGC-1 plasmids from Addgene...

  15. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...Directors brings human resources experience and insight to the now 13 member board at Addgene. Let’s meet...hope Addgene will accomplish in the near future? Right now it’s a matter of maneuvering through the impact...volunteer appointments included the Belmont Human Rights Commission and mediator for the Massachusetts small... dedicated to volunteerism and ran the Mayor’s Midnight Sun Marathon in Anchorage, for the Leukemia Society...

  16. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...to the field. Fortunately, our bodies have been fighting cancer for far longer than we’ve been studying...natural abilities, we’re gaining new ground in the fight against cancer in its many forms. The dichotomous...cancer stops growing, and the immune system stops fighting. Such quiescent cancer may rear its head once ...infection can further divert the immune system from fighting cancer. Surgical removal causes increases in tumor... a sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...

  17. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...instance, you might use it as part of a flex switch you’d like to work with in neurons or you might use it in...Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course...brain. There are many possibilities enabled by this highly modular AAV - that’s probably why it’s so popular...

  18. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...bigly. But such a project should not be undertaken lightly. Whether executed in arrayed or pooled format there...are just as critical for success. Sometimes, you might have a few positive control genes on which to test...execute genome-wide perturbations. So, while some might argue that the only way to do something is to do...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

  19. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out together. “We also ... celebrate your lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes...
Showing: 501 - 520 of 989 results