Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,038 results for: LAS

Showing: 501 - 520 of 1038 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...at least four times more efficient in primary fibroblasts. iTOP also works in multiple types of primary...and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-... which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated recombination...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ... waste of our time.  Networking connections can last years This is a graphic snapshot of my LinkedIn ...graduate school, there was only one other person in my class who had children. When I had my baby, we became ...the postdoc association. It was 25 years since our last contact, but we were delighted to be reconnected...years in many different ways. This leads me to my last tip: It’s usually not worth connecting with people...

  3. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...shyer classmates. For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry...but adamantly refused to get them vaccinated. A classmate told me that they never finish a course of antibiotics...anxious, whispered questions from friends and classmates back in high school, and it’s oddly fitting that...

  4. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors...recently? Most of us at Addgene began working from home last week to flatten the curve, but working remotely ... Many studios and gyms are now offering online classes and participating in these is a great way to support...

  5. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...wavelength of laser needed to excite them - and it’s a good idea to make sure that laser is available ...wavelength of light in response to excitation by a laser, which is then read by a detector panel in the machine...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...adenovirus cargo is high, it’s generally not as long lasting as AAV (up to several weeks vs. AAV’s several years...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Enzymes An equally, if not more, common conjugate class than fluorophores are enzymes. The most common enzyme...clinical applications). But there are a few other classes that we would be remiss not to at least mention...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene...

  12. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

  13. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover is radioactive...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system...

  14. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...starting with the hiring manager. These interviews lasted for 30 minute to an hour. Although I never experienced...prior research project. In total, the interviews lasted from half to all of the day.  This whole process...technical difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a... is complete Industry interviews are long! Mine lasted 4.5 hours, which was a lot of extraversion for ...

  15. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References and Resources References..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  16. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  17. CRISPR in the Clinic

    Type
    Blog Post
    ...underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood disorders Two...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ...introducing a desired edit, but this particular class of treatments has quite the opposite aim: to destroy...ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History ...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions...

  19. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...the complex is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily...a surface format made of rock-hard, inflexible plaster. As a result, the model obscures the flexible nature...Designs web site. Or, if you are visiting a local classroom or trying to explain your science to the general...Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...them in ways that make them much easier. A special class of FPs, the FP timers, add an entire new dimension....  Table 1: Monomeric FP Timers and Associated Plasmids from Subach et al. Protein Excitation (nm) ...) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red...dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP...
Showing: 501 - 520 of 1038 results