We narrowed to 1,039 results for: LAS

...coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend much less time wrangling...helping scientists share information and, of course, plasmids. My primary duties are to manage this very blog...interesting than that will simply get people ordering plasmids. The need to keep content coming also keeps me...talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene can ...

...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...shipping staff agreed to come in and start getting plasmid deposits into the collection and to start shipping...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

...The principle of Tetbow In modern neuroscience, plasmid or viral vector-mediated gene delivery is more ... use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation...Tips and troubleshooting your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have...achieve the highest color variations. Adjust the plasmid concentrations or AAV titer for the best results...

... BRAIN initiative plasmid collection More resources on the Addgene blog Plasmid tools for microbiome...Anikeeva lab are microelectronic fibers made from thermoplastic polymers that can work with all the bells and...authors optigenetically and chemically excited a class of enteroendocrine cells within the intestines known...

...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ...signal to noise ratio five fold over the 1xHA tag.  Lastly, the researchers found that the HA frankenbody ...the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be used for imaging in ...

...at least four times more efficient in primary fibroblasts. iTOP also works in multiple types of primary...and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-... which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated recombination...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

...shyer classmates. For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry...but adamantly refused to get them vaccinated. A classmate told me that they never finish a course of antibiotics...anxious, whispered questions from friends and classmates back in high school, and it’s oddly fitting that...

...have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors...recently? Most of us at Addgene began working from home last week to flatten the curve, but working remotely ... Many studios and gyms are now offering online classes and participating in these is a great way to support...

... waste of our time.  Networking connections can last years This is a graphic snapshot of my LinkedIn ...graduate school, there was only one other person in my class who had children. When I had my baby, we became ...the postdoc association. It was 25 years since our last contact, but we were delighted to be reconnected...years in many different ways. This leads me to my last tip: It’s usually not worth connecting with people...

...wavelength of laser needed to excite them - and it’s a good idea to make sure that laser is available ...wavelength of light in response to excitation by a laser, which is then read by a detector panel in the machine...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

...t require an excitation light source (usually a laser in fluorescence assays), which means luminescence... partnered with Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT...collection page Promega Plasmid Collection Fluorescent Proteins 101 ebook ... plasmids 101...

...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...adenovirus cargo is high, it’s generally not as long lasting as AAV (up to several weeks vs. AAV’s several years...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and...

...mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

...Enzymes An equally, if not more, common conjugate class than fluorophores are enzymes. The most common enzyme...clinical applications). But there are a few other classes that we would be remiss not to at least mention...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene...

... a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover is radioactive...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system...

...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

...starting with the hiring manager. These interviews lasted for 30 minute to an hour. Although I never experienced...prior research project. In total, the interviews lasted from half to all of the day.  This whole process...technical difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a... is complete Industry interviews are long! Mine lasted 4.5 hours, which was a lot of extraversion for ...