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Showing: 501 - 520 of 811 results

  1. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

  4. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell...6276558. 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage ...

  7. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...environmental plasmids, are all around us! A plasmid is a small piece of DNA that can replicate independently from... genetic parasites. Cryptic plasmids tend to be small and poorly studied, but some researchers have suggested...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    ... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...antibiotic screening for selection, failed under normal cycling protocols, and required a 48-hour incubation...

  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...when the antibody is produced from a new set of animals), and wrong application (different experiments/...antibodies. A goldilocks antibody is highly specific (minimal binding to off-target molecules), reliable (wouldn...

  12. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...transitioned between different lab groups without having a formal interview process. Industry interviews, however...structured. I met with several individual and/or small groups, usually starting with the hiring manager...

  15. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...publish brief novel findings. These results are normally one figure and can be negative and/or even replicated...develop computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the... fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence)...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...'t do it unless you can do it well" – even the smallest program. I don't mean have fancy food or a fantastic... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...
Showing: 501 - 520 of 811 results