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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 818 results for: Mal

Showing: 501 - 520 of 818 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response...Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters...

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... attain cell-specific expression in transgenic animals, where cre expression is restricted to a particular... which can then be exclusively activated by the small molecule clozapine N-oxide (CNO) (Krashes et al....

  3. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...preceded by GA or followed by TC) would be cut as normal. If not taken into account, this blocked site could...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...resulting in two independent Fab fragments with small tails of the hinge region. Size: ~55 kDa.  Fab -...handful of advantages over full antibodies. Their smaller size allows them to get into samples more efficiently...

  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

  9. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ...

  10. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell...6276558. 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage ...

  13. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...transitioned between different lab groups without having a formal interview process. Industry interviews, however...structured. I met with several individual and/or small groups, usually starting with the hiring manager...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...environmental plasmids, are all around us! A plasmid is a small piece of DNA that can replicate independently from... genetic parasites. Cryptic plasmids tend to be small and poorly studied, but some researchers have suggested...

  17. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...publish brief novel findings. These results are normally one figure and can be negative and/or even replicated...develop computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid...

  18. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...when the antibody is produced from a new set of animals), and wrong application (different experiments/...antibodies. A goldilocks antibody is highly specific (minimal binding to off-target molecules), reliable (wouldn...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    ... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...
Showing: 501 - 520 of 818 results