We narrowed to 568 results for: PAC;

1. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   ...options for mutually beneficial solutions. If you package your challenges with your supervisor into learning...

2. Neurodegeneration Research Collection

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   Collection

   ...Community (Link opens in a new window) Research workspace on Protocols.io (Link opens in a new window) ....

3. Hot Plasmids: Winter 2025

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   Blog Post

   ...from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). Figure...

4. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...that are distant linearly but close together in space. The chromatin “picture” is then developed by detecting...

6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

8. Transferable Skills Guide: Managing a Team

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   Blog Post

   ...and not valued which will undoubtedly negatively impact your work and your life. It’s up to you to decide...

9. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   ...strain Differences between E. coli host strains can impact plasmid yield. Check the bacterial strain to see...

10. Experimenting with New Careers while in Grad School

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    Blog Post

    ... once a week to get recharged. The fun change of pace helped break up the negativity and allowed me to...

11. Genome-wide Screening Using CRISPR

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    Blog Post

    ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...

12. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...

13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...

14. COVID-19 Resources

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    Collection

    ...neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection -...

15. Great Results Start with Great Standard Curves

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    Blog Post

    ... be using ahead of time to see what factors may impact the dynamic range of the assay. Try to minimize...

16. DNA Purification Without a Kit

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    Blog Post

    ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ...gene function while using fewer sgRNAs. These new compact libraries will allow scientists to conduct genome-wide...

18. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ... is the transferable skills that create a full package and add an extra shine to your work. Knowing your...

19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ...affinity for endogenous molecular targets such as paclitaxel. Unlike other systems, no genetically encoded...

20. CRISPR Antimicrobials

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    Blog Post

    ...containing a phage origin of replication that can be packaged into replication-incompetent phage particles using...