We narrowed to 663 results for: PPL;

1. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   ... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...

2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

   Type

   Blog Post

   ...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

3. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   ...number and reagent description is lost (e.g. a supplier is bought out by another company and catalog number...

4. Pushing the Limits of DNA Assembly

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   Blog Post

   ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...

5. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...

6. 5 Great Apps for Lab Life

   Type

   Blog Post

   ...lab notebook. Lab Archives is a multipurpose application that allows you to share your data with colleagues...

7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...

8. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   ...High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. ...

9. Finding and Joining Your Dream Lab

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   Blog Post

   ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...

10. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    ...your cells divide. Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial...

11. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ...can also allow multiplexing with experiments that supply both substrates and measure luciferase output at...

12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ... above (envelope, packaging, and transfer) are supplied by three types of plasmids, which are cotransfected...

13. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...

14. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

15. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    ...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...

16. Antibodies 101: Isotypes

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    Blog Post

    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    ...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

18. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...

19. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    ...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...

20. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...