... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...

...your own favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently... other species, and libraries using strategies related to other nucleases as well as inhibitory and activating...

...resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple...photons achievable with photoswitchable dyes. Correlative light and electron microscopy (CLEM) is an imaging...

...epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed repair (HDR) to...they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide...

...60 minutes of illumination, highlighting its reliability for long-term experiments (Salari et al., 2019...unconjugated dye molecules are critical for accurate and reliable imaging and assay results (Hayashi-Takanaka et...

...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...

...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently... DREADDs in experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating..., Jin J (2015). The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...460. PMID: 15765260 (Link opens in a new window) Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ...

...two tandem copies of the gRNA cassette, one can reliably knockdown expression of multiple target transcripts...Streptomyces Some of the most industrially and clinically relevant bacterial species reside in the genus Streptomyces...

...the wild-type opsin of each and some examples of relevant variants. This list is not exhaustive - please...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...light-controlled gene expression. These plasmids rely on the engineered two-component regulatory system...

...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

...fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during the school year...

...-specific topic, allowing you to easily access relevant information for planning, executing, and troubleshooting...

...models for disease and open science: Interview with Darrell Kotton Find additional plant biology blog posts...

...rather astonishing ability to...well, annoy. Fly-related Issues in the Lab A significant drawback of working...

...We hope it helps you accelerate your research. Sincerely, Melina & The Addgene Team ...

...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...

...the scientific community. Here are a few virus-related protocol videos that may help to save you time ...

...globe very quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which...

...Cancer and the Immune System: Deciphering the Relationship...

...Fessenden's example of a FRET experiment with all relevent controls near the end of the post also really ...