Transferable Skills: Negotiation
Type
Blog Post
... do next to how much data is necessary before submitting a manuscript. In fact, how many of you have negotiated...
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Academic vs. Industry Postdocs
TypeBlog Post...will be reviewed by an internal committee before submission to ensure that it does not divulge any propriety... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...vast majority of AdV-modified human cells being subjected to scarless homology-directed genome editing (... -
Hot Plasmids: Summer 2025
TypeBlog Post...protein AMPA.FingR (AF) that binds to AMPA receptor subunit GluA1 found at excitatory synapses (Rivera & Huang... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post.... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition to the expected... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum where you... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...-coding sequence using a recoding strategy; a subsequent drive can restore the wild-type sequence (save... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...experiments. Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is ... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...incredible is the bioeconomy is already pretty substantial. It's estimated that genetic engineering as applied... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post... global activity in our particular scientific subfields. Plasmid requests correlate with paper publication... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...use, it’s worth scheduling the second and other subsequent meetings. The number of participants, breadth... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...worrisome for me since bioethics is an inherently subjective area. For CRISPR, I focused on the fact that,... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...genomic locus, which they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for the identification... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...These pages provide helpful information about the subject, link to related guides and protocols, and provide... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post... from Addgene and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed ... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...kind of content are you sharing? Sometimes your subject matter will drive the kinds of communications you... -
Tips for arabidopsis transformation
TypeBlog Post...likely that such a construct will be silenced in subsequent generations. You will know if you obtained a ...