We narrowed to 583 results for: SUB;

1. Pooled Library Amplifications

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   ...Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 20 bp ...

2. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   ...correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish between...

3. PhD Applications After COVID

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   ...that you reach out to potential advisors before submitting your application. I found this step to be incredibly...

4. Transferable Skills: Negotiation

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   ... do next to how much data is necessary before submitting a manuscript. In fact, how many of you have negotiated...

5. Academic vs. Industry Postdocs

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   ...will be reviewed by an internal committee before submission to ensure that it does not divulge any propriety...

6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...vast majority of AdV-modified human cells being subjected to scarless homology-directed genome editing (...

7. Viral Vectors 101: Voltage Indicators

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   ...directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

9. PITChing MMEJ as an Alternative Route for Gene Editing

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   ... anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition to the expected...

10. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum where you...

11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...-coding sequence using a recoding strategy; a subsequent drive can restore the wild-type sequence (save...

12. Which Fluorescent Protein Should I Use?

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    ...experiments. Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is ...

13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...incredible is the bioeconomy is already pretty substantial. It's estimated that genetic engineering as applied...

14. Viral Vectors 101: The Retroviral Lifecycle

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    ...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...

15. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ... global activity in our particular scientific subfields.  Plasmid requests correlate with paper publication...

16. A Guide to Starting Your Own Journal Club

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    ...use, it’s worth scheduling the second and other subsequent meetings. The number of participants, breadth...

17. SciComm: Taking Science to Elementary and Middle Schools

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    ...worrisome for me since bioethics is an inherently subjective area. For CRISPR, I focused on the fact that,...

18. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...genomic locus, which they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for the identification...

19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...These pages provide helpful information about the subject, link to related guides and protocols, and provide...

20. Tips for arabidopsis transformation

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    ...likely that such a construct will be silenced in subsequent generations. You will know if you obtained a ...