We narrowed to 605 results for: She

1. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt...

2. Plasmids 101: Cre-lox

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   Blog Post

   ... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse...

3. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   ...strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard...

4. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...

6. How to Prepare for an Industry Interview

   Type

   Blog Post

   ...lunch on your schedule, but it will probably be rushed and you’ll have to answer questions between bites...

7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   ...work has focused on modifying the outside protein shell, or capsid, of the virus so that it only enters ...

8. Lab Automation at Addgene

   Type

   Blog Post

   ...would be much easier with a Tube Writer, which air brushes the information onto the vials instead of requiring...

9. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   Blog Post

   ...wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody...

10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    ...Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 ...

11. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    ... Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R. M., Kiefl, E., Moody...

12. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

13. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ...on! References and Resources References Nasu Y, Shen Y, Kramer L, Campbell RE. Structure-and mechanism-guided...

14. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    ...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...

15. Engaging with science and society at pgEd

    Type

    Blog Post

    ...as a society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure...

16. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017...

17. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    ... a project forward, transductions should not be rushed into. Before you start any transduction, you should...

18. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    ...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...

19. General Transfection

    Type

    Protocol

    ...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

20. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

    ...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ...