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We narrowed to 927 results for: TIM;

Showing: 501 - 520 of 927 results

  1. dTAG - You're it!

    Type
    Blog Post
    ... and selective degradation of protein targets in time scales that are not possible with traditional genetic... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...papers noted below for examples of dose-responses and time-courses (Nabet et al., Erb et al., Huang et al.,...

  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...scientists frequently use growth factors or serum to stimulate a pathway of interest, but there can be a lot ...occurs when serum or growth factors are used as a stimulus. This system also allows precise modulating of...delayed effects because you can precisely control the timing of kinase activity. 2. Studying negative feedback...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... Don’t Know This Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations...Connect with people you have really talked to The best time is within a few days after you’ve met. Always include...their profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...deep sequencing and qPCR methods are too costly and time intensive to process a large number of microbiome...containing the particle exponentially decreases over time and the number of elapsed generations can be determined...

  5. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...plasmids, viral service, and more For the first time in the last couple of years, we don’t have to worry...way. Scientists know this and recognize it all the time. We love this feedback and are always adding to ...Speaking of the website, we now have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular...

  6. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...antibodies and find….hundreds of options. With limited time and budget you cannot test them all, so how do you...citations. An antibody that has been cited thousands of times may be a safer bet than one that has never been ...free to make the decision by price point, shipping time, or other factors. Just remember that these are ...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ... scientists and has saved countless hours of lab time (in both donating and receiving labs)” — Jim Woodgett...alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally invaluable resource. I...Addgene that has been distributed more than 100 times.   “I really like this award because you don’t get...

  8. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...At the same time, an adjuvant such as KLH or Freund’s adjuvant is injected, which stimulates the overall...that they are inexpensive. Their short production time and the bulk purification steps keep the cost low...

  9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is the ...DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another gene found ...cloning applications. While these two strains ultimately perform the same function, there is some evidence...

  10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...push the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering... need the ability to turn on genes at different times. It requires more complex synthetic networks. Addgene...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

  11. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...in culture for up to 20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw...effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the course...mL of sample. The purity of the sample can be estimated by looking at the ration of DNA (260nm) versus...

  13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...pluripotency marker expression and response to LPS stimulation, respectively. Primary cell type Gene targeted...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... vectors you can find and use them as-is, saving time and effort but risking the potential of them not...vectors (McGee et al., 2024). It reduces the amount of time to design and create a new vector to four days for...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

  16. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...types of people both above and below you. During my time at the NPA, there were many changes to the  Board... As I spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee...research field should I decide to remain here.  In a time when it is so important for postdocs and graduate...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...systems include that virus production can be quite time consuming and that insect cells require demanding...proteins from plants however can be expensive and time consuming as plant tissues themselves are biochemically...cells and growing conditions often need to be optimized. Yeast cells are also known for hyperglycosylating...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference After using your elevator pitch several times at the conference, you might notice some trends....out if you’d like to meet someone and set aside a time to meet. Some conferences may also have a dedicated...there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour) and use the conference hashtag...

  19. Of Myc and Men

    Type
    Blog Post
    ...questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags For about...ideal.  Unexpected connections Right around the same time that Munro and Pelham were devising their tagging...Schüchner, et al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility...

  20. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...or disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role...high-throughput genetic screen, it is expensive and time consuming to individually generate gRNAs and homology...the Addgene Blog Learn about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins...
Showing: 501 - 520 of 927 results