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We narrowed to 936 results for: TIM;

Showing: 501 - 520 of 936 results

  2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... negative selection in plasmid cloning.  But oftentimes, a selection strategy is not enough on its own...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing or the other techniques...

  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... where image processing algorithms for analyzing time-lapse images, quantitatively evaluating antibody...download the software for use, or they can modify and optimize the code, find and fix security-critical bugs,...the world don't have to start from scratch every time to pursue their scientific curiosity but can literally...

  4. Clear and Accessible Writing

    Type
    Blog Post
    ...graduate student using viral vectors for the first time and learning how to purify AAV.  Second, you’ll ... team, working together to share information. Sometimes it is most helpful to use a lot of jargon and ...and have a technical, detailed approach. Other times, it is more helpful to use plain language and focus...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different human...signaling cascades as a response to the extracellular stimuli. Ligand dissociation from the GPCR allows for binding...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

  6. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...tend to ask similar questions, thus devoting some time to writing a solid personal statement will pay dividends...with your personal statement, while you make the ultimate case: why you? 7. Strength in numbers Encourage...clinch every opportunity for themselves. This is ultimately the wrong approach. It is very likely you know...

  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... the zinc finger nucleases. Unfortunately, it is time consuming and difficult for investigators without...genetically modified cells in less than a month's time. Addgene: What are the implications of this kit ...flexibility in terms of the target site. Also, any time you create double-strand breaks, there is a possibility...

  8. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...copy numbers vary within cell populations and over time resulting in variable gene expression that can impact...expression of enzymes in a pathway will ideally be optimized to promote generation of the desired product while...Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains...

  9. dTAG - You're it!

    Type
    Blog Post
    ... and selective degradation of protein targets in time scales that are not possible with traditional genetic... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...papers noted below for examples of dose-responses and time-courses (Nabet et al., Erb et al., Huang et al.,...

  10. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... Don’t Know This Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations...Connect with people you have really talked to The best time is within a few days after you’ve met. Always include...their profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...deep sequencing and qPCR methods are too costly and time intensive to process a large number of microbiome...containing the particle exponentially decreases over time and the number of elapsed generations can be determined...

  12. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...plasmids, viral service, and more For the first time in the last couple of years, we don’t have to worry...way. Scientists know this and recognize it all the time. We love this feedback and are always adding to ...Speaking of the website, we now have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular...

  13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...scientists frequently use growth factors or serum to stimulate a pathway of interest, but there can be a lot ...occurs when serum or growth factors are used as a stimulus. This system also allows precise modulating of...delayed effects because you can precisely control the timing of kinase activity. 2. Studying negative feedback...

  14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is the ...DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another gene found ...cloning applications. While these two strains ultimately perform the same function, there is some evidence...

  15. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...antibodies and find….hundreds of options. With limited time and budget you cannot test them all, so how do you...citations. An antibody that has been cited thousands of times may be a safer bet than one that has never been ...free to make the decision by price point, shipping time, or other factors. Just remember that these are ...

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...At the same time, an adjuvant such as KLH or Freund’s adjuvant is injected, which stimulates the overall...that they are inexpensive. Their short production time and the bulk purification steps keep the cost low...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    ... scientists and has saved countless hours of lab time (in both donating and receiving labs)” — Jim Woodgett...alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally invaluable resource. I...Addgene that has been distributed more than 100 times.   “I really like this award because you don’t get...

  18. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...push the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering... need the ability to turn on genes at different times. It requires more complex synthetic networks. Addgene...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

  19. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...in culture for up to 20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw...effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the course...mL of sample. The purity of the sample can be estimated by looking at the ration of DNA (260nm) versus...

  20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...pluripotency marker expression and response to LPS stimulation, respectively. Primary cell type Gene targeted...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...
Showing: 501 - 520 of 936 results