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We narrowed to 1,076 results for: TOR

Showing: 501 - 520 of 1076 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...researchers, it can expose them to a new institute and mentor that they may want to consider for future work ...professor or institute, ask if you will have a bench mentor or anyone else available to directly help you during... during your visit. Reach out to your bench mentor (if you have one) to communicate your plan as well.... comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying...techniques. Show up on time (don’t make your bench mentor wait on you!), make sure you are prepared (read...

  2. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...elicit an immune response from the body. Several factors contribute to the immunogenicity of a drug, including...these algorithms to model individually-specific factors, such as previous exposures to that specific antigen...assessments, as well as analyze the contributing factors that in silico prediction algorithms fail to capture...10.1634/theoncologist.2016-0061WHO. International monitoring of adverse reactions to drugs: adverse reaction...WHO Collaborating Centre for International Drug Monitoring, Uppsala, Sweden, 1992. Mattei, A. E., Gutierrez...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...will choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously... does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager, researcher, adviser...experiments and grant writing. You have to enjoy the mentoring role.” She points out that new PIs may not recognize...student or postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ... are three main pathogen clearing functions or effector functions of the constant regions of antibodies...functions: Recruitment of immune cells that express receptors that recognize the Fc portion of different antibody...domains, or Fc region, can be bound by special receptors that transport antibodies through cells and into...role in allergies. They are found bound to IgE receptors on the surface of basophils and mast cells, two...of an immune response since it also changes the effector function of the antibody. Isotype switching is...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989...be individually optimized for each of the three factors: the ncAA, the tRNA, and the tRNA synthetases. ...balance can be extremely tricky to find: each of the factors can negatively impact both translation and fidelity...complicated.” And Ryan Mehl would know. He’s the director of GCE4All, an NIH-funded center located at Oregon... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...professional network such as co-workers, professors, and mentors. Ask for specific guidance on grammatical errors...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...practice a set of common questions with a peer or mentor. Several days prior to the interview, inspect your...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...

  8. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...gRNAs that can be packaged together in a single vector. In addition, the pol III promoter is relatively...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...tips can help:  If there’s a teacher, mentor, or other educator working with your audience, ask! They ...refers to the work put into creating a permanent store of knowledge (a schema).”  In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...knowledge and expertise your audience has is a major factor in how quickly they can understand and process ...

  10. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...credit to. Thankfully, as Addgene's resident blog editor, I could take the needed steps to continue where...alternative reagents have reduced immunogenicity. Effector functions are eliminated, as is antibody recognition...constant region. Nonspecific binding, caused cell receptors for the constant region, is also reduced.  Disadvantages...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...allowing for them to be used as intracellular inhibitors.  Aptamers are not proteins at all. These high-affinity...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to track telomere...given chromosome simultaneously, they could also monitor the spatial relationship of the two genes over ...

  13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...contributed by guest blogger, Gary McDowell, executive director of Future of Research. On December 1st 2016, many...of Information Act (FOIA) requests for their postdoctoral salary information as of December 1st. Salary...are an important step forward in helping the postdoctoral population continue to pursue academic work,...of the scientific enterprise by reducing the postdoctoral population which keeps growing despite a lack...McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. He is interested in advocating...

  14. All in a Twist: dsRNA

    Type
    Blog Post
    ... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...sword – balancing antiviral benefits with the inflammatory response associated with the introduction of...of duplexed RNA Mammalian cells have multiple receptors in place to sense dsRNA and respond accordingly...threshold and thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated...its way outside of the mitochondria, the same inflammatory response can activate. These types of cellular...

  15. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... all have a PR-T4 vector backbone, which is a modified version of L440. This vector is versatile in that...of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P, ...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly of multiple Level 0 parts into...

  17. Hot Plasmids February 2024

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...

  18. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...2002). Insect cells as a factory to produce adeno-associated virus type 2 vectors. Human Gene Therapy, 13...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV Titers: Where Do They...platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology,...

  19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...microscopy, by cloning your protein of interest into a vector encoding a fluorescent tag. Once expressed as a...golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular ...bacterial components. For example, the Rainbow Vectors from the Mariette Barbier Lab can be used to fluorescently...Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka...Barbier, Mariette, and F. Heath Damron. "Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling...

  20. PhD Applications After COVID

    Type
    Blog Post
    ...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...almost as daunting as the program itself. Picking a mentor comes with many challenges, and the flood of interview...more complex. “How do I reach out to potential mentors?” “Where do I start with picking universities?”...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...
Showing: 501 - 520 of 1076 results