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We narrowed to 888 results for: TRI

Showing: 501 - 520 of 888 results
  1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel... the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD...
  2. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...
  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families...
  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...
  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...
  6. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona...governed how this game was played. These rules restricted which cards could be played when and, depending...
  7. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...
  8. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...
  9. How Addgene Creates Content

    Type
    Blog Post
    ...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...
  10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...
  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...
  12. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...
  13. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...
Showing: 501 - 520 of 888 results