We narrowed to 888 results for: TRI

1. Donations from Addgene to Yield Answers for Rare Disease Researchers

   Type

   Blog Post

   ...submissions from 99 universities and foundations in 21 countries. “Over $600,000 worth of cutting-edge technologies...with one form of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different...

2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   ...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel... the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD...

3. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   ...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...

4. Celebrate National Book Month with Addgene

   Type

   Blog Post

   ..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families...

5. New and Upcoming Viral Vectors - June 2019

   Type

   Blog Post

   ...help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...

6. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

   Type

   Blog Post

   ...“leaf” with similar permeability of water and nutrients and colonization opportunities for bacteria. To...the control of promoters responsive to water or nutrient levels can help him determine whether the artificial...

7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

   Type

   Blog Post

   ...Apoptosis is a highly controlled process that is triggered by internal and external signals such as developmental...new protein-protein interaction sensor based on tripartite split-GFP association." Scientific reports 3 ...

8. Revamp Your Lab Meetings With Creative Virtual Collaboration

   Type

   Blog Post

   ...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...built-in whiteboard, in which participants can contribute and sketch on the board without leaving Zoom....

9. "Build Your Plasmid": The Game - Play to Teach

   Type

   Blog Post

   ... This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona...governed how this game was played. These rules restricted which cards could be played when and, depending...

10. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...

11. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    ...This post was contributed by Kelly Hills, founding bioethicist of Rogue Bioethics. On November 25, the...animal models, and several stages of human clinical trials. This model also demonstrates that when we are ...

12. R Bodies: Membrane-Rupturing Microscopic Tools

    Type

    Blog Post

    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...

13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

14. How Addgene Creates Content

    Type

    Blog Post

    ...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...

15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    ...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

16. Communicating Your Science With Help From ComSciCon

    Type

    Blog Post

    ...This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference ...employer that your past work has prepared you to contribute at a high level to their team.  When you're writing...

17. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors...

18. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

19. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    ...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...embargoed while the research is ongoing, but all registries do require that research plans become public ...