We narrowed to 577 results for: Vit

1. Addgene's Cancer Collection Pages Connect the Research Community

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   ...to include several other important pathways. We invite feedback and contributions from all members of ...

2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...

3. Plasmids 101: Multicistronic Vectors

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   ...RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...releasing simply plug into this existing economic activity. People are using BioBrick parts to make more ...

5. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ... last decade. He describes the detection of gravitational waves--a story I was much less familiar with...

6. An Introduction to Adenovirus

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   ...and rapidly caused the death of adenoid cells in vitro (Rowe et al., 1953). While overt death of the targeted...

7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...membranes and permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize...

8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms a novel co-complex with the...

9. Simplify Cloning with in vivo Assembly

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   ...techniques involve the assembly of a circular plasmid in vitro, before transforming it into E. coli for propagation...

10. Plasmids 101: Protein tags

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    ...various mutations used to increase its stability activity, can be readily purified using plasmids found ...

11. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...groups. What apps do you use to improve your lab productivity? As this is a growing market, I am sure I missed...

12. Negotiating Work and Life: How to Find the Joy

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    ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...

13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ... as increased specificity and lower off-target activity (Hu et al., 2018).  In 2020, the Kleinstiver lab...

14. An Inside Look into Addgene's Dev Team

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    ...to increase all of our teams’ efficiency and productivity. Get to know our software development team and...

15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...mice strain specific Ly6a SNPs on PHP.B binding in vitro. They found that when LY6A contained C57BL/6J SNPs...

16. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...regulatory regions that are necessary for myosin activity and thus interaction with actin in nonmuscle systems...

17. Designing Your Chalk Talk for the Academic Job Interview

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    ... seek to convey in your chalk talk, there's the vital task of engaging your audience. You need to make...

18. Lab to Office Culture Shock

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    ...of the nature of your time course, but you’ll inevitably have downtime and plenty of it. That’s not the...

19. CRISPR-mediated Plant Base Editors

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    ...2018) (see CBE-3 in Figure 3). Figure 3: Activity windows of plant base editors. Plant CBE and...

20. Viral Production at Addgene

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    ...Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function....