We narrowed to 573 results for: Vit

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...releasing simply plug into this existing economic activity. People are using BioBrick parts to make more ...

2. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ... last decade. He describes the detection of gravitational waves--a story I was much less familiar with...

3. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ...techniques involve the assembly of a circular plasmid in vitro, before transforming it into E. coli for propagation...

4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   ...nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms a novel co-complex with the...

5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...membranes and permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize...

6. An Introduction to Adenovirus

   Type

   Blog Post

   ...and rapidly caused the death of adenoid cells in vitro (Rowe et al., 1953). While overt death of the targeted...

7. Plasmids 101: Protein tags

   Type

   Blog Post

   ...various mutations used to increase its stability activity, can be readily purified using plasmids found ...

8. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...

9. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

   Type

   Blog Post

   ...Karmakar et al., 2024; Karmakar et al., 2025). We invite researchers to explore and use our TnpB-based genome...

10. Hot Plasmids: Fall 2024

    Type

    Blog Post

    ...degraders. But CRBN has been difficult to study in vitro, requiring either expressing it with additional ...

11. Hot Plasmids Spring 2024

    Type

    Blog Post

    ...They demonstrated REVeRT in various contexts in vitro and in vivo, including in human retinal organoids...

12. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    ...you stand out.  In some fields, a cover letter is vital, particularly in fields where communication and ...

13. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works...

14. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...for discussions and potential collaborations. We invited experts from other universities and maintained ...

15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ... as increased specificity and lower off-target activity (Hu et al., 2018).  In 2020, the Kleinstiver lab...

16. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    ...to increase all of our teams’ efficiency and productivity. Get to know our software development team and...

17. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging...

18. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ...regulatory regions that are necessary for myosin activity and thus interaction with actin in nonmuscle systems...

19. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ... seek to convey in your chalk talk, there's the vital task of engaging your audience. You need to make...

20. Lab to Office Culture Shock

    Type

    Blog Post

    ...of the nature of your time course, but you’ll inevitably have downtime and plenty of it. That’s not the...