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  2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular...

  3. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from...properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. ...

  4. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines... ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to correctly read through...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...CAR–T cells was recently devised by the Yingxiao Wang lab.  The crux of the system is a set of heat-inducible...

  8. The MTA at Addgene

    Type
    Blog Post
    ...or agreement that states the terms of use for a tangible research material that is shared between two parties... via an online process, though the process has changed a lot over the last twenty years! This has helped...

  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    .../fddsv.2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, ...https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic...

  10. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... reagents readily available. What if that could change? Everyone in science needs both a job and funding...around 100 professional editors who produce a broad range of primary research and review journals across life...

  12. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  13. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

  14. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

  18. Antibodies 101: Isotypes

    Type
    Blog Post
    ...switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ...

  19. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral integration... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...
Showing: 501 - 520 of 827 results