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We narrowed to 835 results for: ang

Showing: 501 - 520 of 835 results
  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and allowing ...mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology doesn’t have to...
  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...
  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... reagents readily available. What if that could change? Everyone in science needs both a job and funding...around 100 professional editors who produce a broad range of primary research and review journals across life...
  4. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...
  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular...
  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from...properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. ...
  7. The MTA at Addgene

    Type
    Blog Post
    ...or agreement that states the terms of use for a tangible research material that is shared between two parties... via an online process, though the process has changed a lot over the last twenty years! This has helped...
  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    .../fddsv.2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, ...https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic...
  9. Hot Plasmids - January 2023

    Type
    Blog Post
    ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...CAR–T cells was recently devised by the Yingxiao Wang lab.  The crux of the system is a set of heat-inducible...
  10. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...
  11. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...
  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...
  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...
Showing: 501 - 520 of 835 results