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We narrowed to 954 results for: app

Showing: 501 - 520 of 954 results

  1. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...can firmly gain a handle on understanding and appreciating their biology. The importance of psychrophilic... the empty vector. And thus was born ELSA - our happy lil’ cold-tolerant E. coli! Additionally, we’re ...re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a...

  2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

  3. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...teams around the world who combine engineering approaches and scientific know-how to design/build/test ...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system...multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate ...

  5. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation...but there are several hazards to navigate when mapping out a new project. We’ve shared some of the concerns...

  6. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... I will describe some of the many ways you can approach finding someone to give you advice and guidance...see someone with a great professional presence, approach them for advice on what makes them that way. Is...organization has a mentoring program, consider tapping into it and helping make it work by being an educated...

  7. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... work on our external website appearance and functionality are happening and this strengthens our image...support more new projects. Diversification is happening. The Addgene team will have some fun challenges...

  8. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but it’s toxic to tissues...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-...

  9. dTAG - You're it!

    Type
    Blog Post
    ...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective...that are not possible with traditional genetic approaches. In addition, the dTAG system is versatile and...update with vendor and catalog numbers. As we are happy to share aliquots of dTAG-13 in the meantime, please...

  10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... changes in neural activity when paired with appropriate fluorescent optics. Existing red fluorescent ...organisms such as E. coli and yeast. Because this approach uses standardized parts, it allows for greater...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

  12. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...rates for AAVs are generally high enough for this approach to work, although it’s less effective in vivo ... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

  13. The time and cost required to make a plasmid

    Type
    Blog Post
    ...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of...method is free for you, and many labs are quite happy to share, labs that get high volumes of plasmid ...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

  14. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced...times) into the genome. Make sure to choose an appropriate promoter to express your cDNA, depending on the...flavors of activators which can be used with this approach - read more about them on our CRISPRa blog! ...

  15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some... after less than a minute of exposure to the appropriate light. The photoactivatable counterpart to this...protocols to get your CRISPR experiment moving. Happy editing! References and Resources References Hendel...

  17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...and Western Europe have taken three strategic approaches to gender equality over the past several decades...Australia worked with stem cells in mice. They appropriately used male and female mice (using both sexes ... the sex of the most commonly used cell lines appearing in American Journal of Physiology-Cell Physiology...

  18. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... Tang and Cepko were eager to see if they could apply GFP regulation to other types of proteins. They ...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...tail clip, or toe clip) and using PCR with the appropriate primers to detect your targeting vector or gene...your parental mouse should be hemizygous at the appropriate allele. When crossed to a mouse that is homozygous...F1 heterozygotes to each other will generate approximately 1 in 16 mice that harbor homozygous alleles ...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...Scientists are currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal... 2019. For more information about the aims and approach of CeNGEN, check out their publication.   3’UTRome...that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...
Showing: 501 - 520 of 954 results