We narrowed to 912 results for: ire;

...RNA, a fast, strong protein denaturant would be required- something that broke down RNases before RNases... effective at isolating total RNA, this method requires a lot of time and depending on how many samples...without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

...because “it kinda sucked to work remotely. I was also tired of the contracting life where you do a little bit... was helping cover social media while the Hutch hired a full-time social media manager. “I usually start...magazine. Getting pulled in all of these different directions is sometimes at odds with the space needed to...

...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on Women’s Health, emphasizes...gender in science and technology and currently directs the EU/US Gendered Innovations in Science, Health...

...with multiple inserts or internal mutations that require more than two sequencing reactions. In the more...to ensure uniformity of the culture. Sequencing directly from the glycerol stock saves us the step of purifying... Each sequencing service will have different requirements for volume and concentration, and not all providers...

...new job skill? Finish your thesis? Knowing your desired outcomes will help you assess if the coach you ...coach usually will be a clear communicator and make direct suggestions for improvement and change. Work ...relationship has been successful – what are the desired outcomes? Ask openly for clear information on...

...asymmetry in the core sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT...reversed.   Deletion: If the sites face in the same direction, the sequence between the loxP sites is excised...cerevisiae FLP-FRT recombination system is another site-directed recombination technology very conceptually similar...

...laboratory, a high level of experimental control is required, because seemingly tiny external factors can have...overexpress a gene that alters neuronal function directly such as receptor subunits or neurotrophic factors...smaller and may be preferable if wider diffusion is required. On the other hand, lentiviral vectors are superior...

...Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data on all the cell divisions of ...Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap...Project: The Complete Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed...

...active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is... is expected to contribute to experiments that require high photostability, such as time-lapse imaging...Leopold Parts. This library delivers 2 randomly paired guides per construct and allows a reduced scale...

..., comprised of PAS and GAF domains of iRFP713. Requires presence of heme oxygenase for chromophore formation...miRFP709 and miRFP670, pair with common PAS domain. Requires presence of heme oxygenase for chromophore formation...BiFC interaction is typically irreversible and requires some time for chromophore maturation, splitFAST...

...viral infection by bacteriophages), or conjugation (direct transfer from one cell to another). Bacteria need...transfer genetic material to a recipient through direct contact. That’s conjugation! F factors can also...cloning. In addition, scientists have engineered entirely new types of plasmids without clear analogues ...

...simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For example...number in the Level 2 assembly. These pathways can require a lot of optimization - which is why MoClo is so...of a combination of multiple genes to achieve a desired effect. This is commonly used in plant biology ...

...Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived cells can alternatively...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...inflammatory diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR...

...longer incubation times and didn’t necessarily require cycling. The Pros and Cons Researchers used the...select for correctly assembled phage genomes, it required a very stringent selection process and benefited...selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While the 52-piece...

...Targeting Receptor Expression Using chemogenetics requires successfully introducing receptors to target cells... to control ion flux. The system allows you to directly manipulate ion channels. When working with PSAM...Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson...

...photon 2. Energy transfer from the excited donor directly to the acceptor fluorophore--think of it as a ...fusions varies with transfection efficiency and any unpaired fluorescent proteins can contribute additional...Check Out Fluorescent Protein Biosensors Browse the Entire Fluorescent Protein Collection ...

...effectively utilize the host cell’s inherent homology directed repair (HDR) process following the double strand...break and introducing the tag through homology directed repair To introduce the fluorescent tag to the...on the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining...

...that the orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or ... the antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target... Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination...

...world. With three Nobel Prize winners, one NIH Director and a co-founder of BGI-China presenting, it’s... discoveries and moving beyond DNA Eric Green, Director of the National Human Genome Research Institute...proteins). The chemistry is complex and this work requires an interdisciplinary team.  Day two of the conference...

...alternative approach to NHEJ is to utilize the Homology Directed Repair (HDR) to knock in corrective genes. For...to repair the double strand break via homology directed repair and you would probably need to use a two...human cells being subjected to scarless homology-directed genome editing (Maggio et al., 2014). Gonçalves...