Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars

Showing: 501 - 520 of 971 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...are responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation... scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from... pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. Lastly, the ...FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

  2. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...FPs are tremendously complicated entities with a large range of characteristics (color, brightness, photostability... a project written in Python & Django, using a popular database management system (PostgreSQL), that aims...use case for FPbase is finding information on a particular FP; this can be done directly using the search...your help!), I believe this represents one of the largest available online collections of FP spectra. Every...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...the cell, and potentially spread out over an even larger area, thus necessitating even higher retrograde...methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant adeno-...Adeno-associated Virus Transduction and Integration. Molecular therapy : the journal of the American Society ...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology, genetic engineering... He is also founder and consultant at FRESCI particularly interested in advanced in vitro models technology...Resource Center Resources on Addgene.org Find molecular biology educational resources Find protocol videos...

  5. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...orchestrated movements of networks of proteins consuming molecular fuel sources. While the importance of understanding... realized that these mutations clustered in a particular region of the R-body. Further mutatgenesis of...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...

  6. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...contain all fluorescent proteins in common use (particularly not the most recently published ones), they ...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission than the mCherry...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...

  8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable by the...terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid...

  9. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

  10. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos were designed with...

  11. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...scientists who have gone the extra mile for this particular project. John Doench and David Root from the ...

  12. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

  13. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...biobuilder.org) or by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub...for all who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...is a member of the curriculum in Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics...Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

  15. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ... the inability of an organism to synthesize a particular organic compound required for its growth. Many...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 is synthesized...active enzyme. Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy...

  17. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...setup we can observe the whole population of one particular protein in neurons or in any other cell type....attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...This system also allows precise modulating of a particular arm of the signaling pathway (i.e. everything...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Determining which features to verify will depend on the particular plasmid you are working with, but, in most cases... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...forward and reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...
Showing: 501 - 520 of 971 results