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We narrowed to 968 results for: lat

Showing: 501 - 520 of 968 results

  1. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without...agrochemical compounds that have the potential to regulate the parasitization of cereal crops. As a result...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...

  2. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    [email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Team! Why MTAs Matter Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they...

  3. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ..., increasing the impact of the work, and as a collateral benefit, increasing the impact of the specific...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...Root System Architecture of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at ...expression patterns in plants with enhanced sodium accumulation in their roots and (3) development of tools ...

  4. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling and structure-guided...which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and emission...combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1...fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID...

  5. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several... neuroscience was carried over and allowed to percolate within a new intellectual environment. Over time...perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental ... health policy implications arising from sport-related concussion.   More resources on the Addgene blog...

  6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...multiple, small colonies After streaking LB agar plates, you may notice that there are both large and small... bacterial cultures with lentiviral vectors, the flat white colonies contained the full plasmid, whereas...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. RNA 25:507–518. https...

  7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ... encode spikes found on all of the different circulating variants. This allowed us to create pseudoviruses... the vaccine, we've been able to measure their relative ability to neutralize.”   Figure 2: Sera... shocked [with these results] because we were simulating the live virus experiment,” says Balazs. “But...resources on the Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources...

  8. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...Careers has developed a web-based, career-planning platform called the Individual Development Plan (IDP) and...using multidisciplinary, collaborative, and translational approaches for scientific tool and technique... of the project. For example, how can future regulations impact your work? What design variables need ...Patent process Business Models Communication Regulatory knowledge Networking Blogging Writing...

  9. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...thought there was room for improvement and a year later it became quite clear that others thought the same... piñata burst, value was subjectively ascribed relative to the basic needs of each individual; the  community...newer tools may still prove somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target...tools allow the user to save the results and return later or share them. Community exclusivity: Different...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... arrangements may affect nuclear processes. The latest of these visualization tools is CRISPR-Sirius, ...specific loci based on the guide RNA sequence. A year later, they developed CRISPRainbow (Ma et al., 2016), ...chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow labeling... proteins. (B) CRISPR-Sirius used to visualize relative locations of the subtelomeric region of the pericentromeric...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    ...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

  12. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...these small DNA strands can form concatemers in later steps. This new NEB kit includes a step that both...the DNA and prevents it from forming concatemers later. The "FS" stands for Fragmentation System and this... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al., 2022...therapeutic antibody itself or as impurities in the formulation. Efforts to predict T cell epitopes focus on ..., and can be used as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes...

  14. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...tried to find an app that could help me with calculations. This is how I came across the Gibco Cell Culture...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

  15. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options... are easier to use across multiple expression platforms: transfection, lentiviral transduction, and AAV...Multiplex gene editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple...

  16. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... was the molecule of interest (analyte), and accumulation of ATP activated GFP expression (receptor), ..., then the transducer could be a microscope, plate reader, or any other instrument which measured GFP ...advantages over other mechanisms. For one, many viral platforms, such as AAV or retrovirus, can either permanently...Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...of your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...expression control Researchers are always wanting to manipulate gene expression – turn it up, turn it down, knock...PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the gene therapy landscape. Sig Transduct...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    ...of interest (4) isolated your antibody:protein:DNA complexes and finally (5) isolated the DNA from your...DNA are no longer bound, and the DNA can then be isolated and studied through a variety of downstream methods...downstream analysis via any methods suitable for isolated and purified DNA, such as sequencing or qPCR. ...

  19. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... to glance at as you work. Draw your plate maps onto your plates, alongside your physical copy. If you...reminders on a sticky note   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental...example, setting up your pipette tips to match your plate layout can help you track samples as you add them...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated regions — on gene...Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was first cloned in ...gene expression. The regulatory element is cloned upstream of the luciferase gene, and expression of luciferase...
Showing: 501 - 520 of 968 results