We narrowed to 802 results for: mak

1. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ... she couldn’t run enough workshops by herself to make the biggest impact. When she spoke with Teytelman...visualization. Not everyone in the group was able to make it physically to instruct the workshop at the conference...

2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   ...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ...exposure to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy... shown in Sargsyan et al. It is also possible to make Rosella fusions and thereby monitor autophagy of...

4. CRISPRainbow and Genome Visualization

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   ...However, they note that certain improvements could make CRISPRainbow even better. Adding an additional hairpin...activation/repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow ...

5. Summer SciComm: Preprints

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   ...comment online! Want the general public (say, policymakers or community organizations) to use your work...those plasmids alongside a journal article, but it makes the research materials available to the community...

6. Summer SciComm Series: Cognitive Load

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   ...time to read the book.  Intuitively, this probably makes sense. It’s a lot easier to read a formulaic fiction...happens to us all!) and offer a variety of options to make your content more accessible to your audience.  ...

7. Antibodies 101: Affinity Reagents

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   ...short linker peptide. The lack of constant region makes them less immunogenic, but they have lower affinity...produced in bacteria and are generally cheaper to make than antibodies. Minibodies are created by binding...

8. What's New in CRISPR - November 2021

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   ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

9. Viral Vectors 101: An Introduction to AAV

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   ...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

10. Plasmids 101: The protein expression toolbox

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    ...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...

11. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...opsin-expressing cells). For other things to keep in mind when making fluorescent protein fusions, check out Joachim ...Fluorescent Proteins 101: GFP Fusion Proteins – Making the Right Connection.     Thank you to our guest...

12. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...is no small task. As a scientist, it is useful to make literature searching and reading a regular part ...Keeping up with the literature There are a few ways to make sure you are keeping up with the literature in your...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...When it comes to making the genetic manipulation, there are two main decisions to make: 1. Which modification...

14. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...off of a circular plasmid via PCR. Typically, to make these constructs, developmental biologists have ...please write to [email protected] and if you make new plasmids for investigating different planarian...

15. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    .... The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism for the project because...PubMed Central PMCID PMC232622. 6. Winzeler, D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K.,...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

17. CRISPR in the Clinic

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    ...effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then the treatment will...systems as well as improving delivery strategies to make fast, effective, and targeted therapy possible. ...

18. Plasmids 101: Modular Cloning

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    ... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

19. Progress Towards a PAM-Free CRISPR

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    ...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...

20. Hot Plasmids and Viral Preps - March 2021

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    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...receiver cells. There are over 40 trillion cells that make up the human body and G-baToN provides versibility...