We narrowed to 622 results for: set

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity...

...found to have a point mutation in the puromycin cassette that makes it less efficient in some cell lines... It contains a point mutation in the puromycin cassette that makes it less efficient in some cell lines...

...RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and ...and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit...

...biochemistry department at the University of Massachusetts Medical School. We are working with postdocs...to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary...

...then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations in...

...carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use...Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher titer...

...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... hybrid gRNA library deletes human alternative cassette exons and disrupts genes with alternative exons...

... website so scientists can view public circRNA datasets and download the custom python scripts needed ...phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to replace the drug...

...while lentivirus requires a BSL-2 setup. This requires a less involved setup when working with mice and slightly...

...different names depending on your flow cytometer’s setup. For instance, BV510 and AmCyan have almost identical...practice, you may be able to do your compensation setup on your experimental samples without needing to ...

... used to compare between different experimental setups. A colocalization analysis example is given in ...detection of one fluorescent signal in a channel setup for a different fluorescent signal) and photobleaching...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are...

...). Misek and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic...by bias within the design of the experiment or dataset itself. It is important for scientists to not only...

...doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral Vector Pseudotypes...Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002) Lentiviral vectors pseudotyped with ...

...GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across 8,792 organisms...biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...doi.org/10.1016/0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid...

..., and are therefore able to infect only a small subset of the cells used in a given experiment. For this...Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017...

...complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble fragmented...2017) CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756...