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  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are...

  7. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...different names depending on your flow cytometer’s setup. For instance, BV510 and AmCyan have almost identical...practice, you may be able to do your compensation setup on your experimental samples without needing to ...

  8. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...). Misek and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic...by bias within the design of the experiment or dataset itself. It is important for scientists to not only...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across 8,792 organisms...biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral Vector Pseudotypes...Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002) Lentiviral vectors pseudotyped with ...

  11. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...doi.org/10.1016/0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid...

  12. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ..., and are therefore able to infect only a small subset of the cells used in a given experiment. For this...Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    ... Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the best tool to use...delivery indicates ~7-21 days are required for the onset of gene expression. However, it has yet to be determined...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...photoactivatable CRISPR gene activation system, but settled on a different optimal fusion protein combination... the protein’s ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the...
Showing: 501 - 520 of 609 results