We narrowed to 536 results for: val

1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   .... See Fowler et al for advice on choosing and validating a selection system. You can test the effect of...

2. CRISPR 101: Homology Directed Repair

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   ...Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR...

3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...single amino acid change (from aspartic acid to valine at position 64) which is sufficient to render the...

4. Hot Plasmids: Summer 2024

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   ...et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab has developed a new...

5. Antibodies 101: Beyond Surface Labeling

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   ... 101: Flow Cytometry Controls Antibodies 101: Validation...

6. An Integrin Antibody Toolkit from IPI

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   ...RGD motif compared to those binding the Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer...

7. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   ...metabolite in glycolysis, so this sensor will be a valuable tool to study cellular physiology and metabolism...

8. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   ...time, Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This strategy allows...

9. Evolution of Lab Techniques

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   ...and sequencing gels, as well as their modern equivalents and see what we can learn from their evolution...

10. Thoughts on the Future of Research 2015 Boston Symposium

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    ...individual who has received a doctoral degree (or equivalent) and is engaged in a temporary and defined period...

11. Plasmids 101: Common Lab E. coli Strains

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    ...Natural resistance Primary Use Genotype ccdB Survival 2 T1R   TOP10 derivative. For propagating plasmids...

12. Viral Vectors 101: Optogenetic Tools

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    ...receptor is activated.  Similarly, PTLs are special, covalently linked molecules (i.e. photoswitches) attached...

13. CRISPR 101: Off-Target Effects

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    ...CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit          ...

14. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based therapeutics....

15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...BRAF gRNA viruses, but you can find a list of validated gRNA plasmids on our website. There are a few ...

16. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current opinion in structural...

17. Transferable Skills Guide: Teamwork

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    Blog Post

    ...Major Qualifying Project (or MQP, the school’s equivalent of a senior thesis), I knew early on that I wanted...

18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ...assays is to find (or generate in-house) a pre-validated antibody for each target protein. To study 20,...

19. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...model organisms and backgrounds, MoClo can be invaluable for more complex editing and screening experiments...

20. Which Fluorescence Microscopy Techniques is Best for Me?

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    Blog Post

    ...objects simply vanish into noise. Due to its unrivaled contrast, fluorescence imaging has emerged as ...