...and makes your experimental setup less cumbersome. 3. Portability across multiple systems Terskikh et al...PMID: 19889833. PubMed Central PMCID: PMC2801723. 3. Verkhusha, Vladislav V., et al. "An enhanced mutant...Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch Up On Your...

...propagation along dendrites for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the ...microscopy at depths up to 600 µm. Figure 3: ASAP5 for voltage imaging. A) ASAP5 selected for ...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

...the gel and add gel components to polymerize. Step 3: digest the sample to allow for even expansion.  ...:832–863. https://doi.org/10.1038/s41596-018-0117-3 Wassie AT, Zhao Y, Boyden ES (2019) Expansion microscopy...applications and there’s just not much to be done about that.  Second, expansion introduces distortions....

...been trafficked between locations). Figure 3: A) LOV-Turbo becomes active when illuminated with... h, and PMA activation induces cFos::GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

... from and how the idea got off the ground Chapter 3 - Addgene as a leader in standardized tech transfer...repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this ...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge...

...not immunogenic, a necessity for in vivo studies; (3) is compatible with single gene KO and pooled screening...faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment that exists in ...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...

...the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649     ...Of course, all of you toiling away in laboratories this holiday season want the work you are doing to... has to do with online accessibility. It is now about as easy to find a paper in a lesser-known journal...

...Improved Vectors for Gene Delivery. Curr Gene Ther 5(3), 299–310. PubMed PMID: 15975007. PubMed Central PMCID...Concepts in the pathogenesis of rabies. Future Virol 3(5), 481–490. PubMed PMID: 19578477. PubMed Central...viral vectors is serotype-dependent. Gene Ther 20(3), 348–352. PubMed PMID: 22418061. PubMed Central PMCID...to its retrograde axonal transport. J Cell Biol 82(3), 798–810. PubMed PMID: 92475. Taymans, J. M., Vandenberghe...usually severely impaired, and only few reports exist about dye molecules that are transported from one neuron...

...up the experiment. The first way, it will take you 3 days to complete the entire experiment, but you’ll...be quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time for...post useful. After a bit of research and thinking about my own processes, I’ve broken down time management...task, you’re actively prioritizing. In my anecdote above, I prioritized splitting cells over meeting up with...was too late. I didn’t spend enough time thinking about the possibilities and how to prioritize them. When...month, or even the year, you should be thinking about two main things: How useful the task is (its utility...plasmids to send to Addgene). Following my advice from above, you should probably take the second option. Even...

...understand your proposal to give you proper consideration. 3. Present an easy to follow seminar See item 2. Attendees...? How will your approach resolve this problem? (2-3 sentences) What will be the focus of your first... potential colleagues study. Think about how you might collaborate or, at the absolute minimum, can appreciate...the first sentence or two what your research is about and what you think you can accomplish. No matter...your seminar accessible. Let the experts ask you about the details and then you can display your deep knowledge...expert's question than to leave everyone confused about your research because you waded too deep into the...simple in principle The first question I usually get about chalk talks is, what is a Chalk Talk? It is a 60...