We narrowed to 643 results for: ATO

1. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

2. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

3. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

4. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...Blouin-Tavel, V., Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R.,...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...

6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold. With an NGG PAM, xCas9...

7. Transferable Skills Guide: Managing a Team

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   Blog Post

   ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

9. Academic vs. Industry Postdocs

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   Blog Post

   ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

10. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

11. Plasmids 101: Protein Expression

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    Blog Post

    ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

13. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...

14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

15. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

17. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

18. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

19. MXS Chaining

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    Blog Post

    ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

20. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...