Addgene

We narrowed to 836 results for: Tat

Showing: 781 - 810 of 836 results

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Plasmids 101: The protein expression toolbox

Type

Blog Post

... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...
All in a Twist: dsRNA

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Blog Post

...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...
Giving gRNAs a Facelift - Synthetic and Beyond

Type

Blog Post

...as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are even going...
Developing Lab Management Software for Biology

Type

Blog Post

... track the location, quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene...
Gendered Innovations: Why Does Sex of the Cell Matter?

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Blog Post

...Gendered Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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Blog Post

... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...ratio for the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...without clearly testing whether a certain methylation state causes a condition. To understand mechanisms and...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...
Plasmids 101: Modular Cloning

Type

Blog Post

... allows users to test a variety of different orientations and components within the system to maximize...
CRISPR in the Clinic

Type

Blog Post

...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...that require high affinity binding, such as immunoprecipitations. Your sample species should also be considered...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

... to a target cell by reducing the repulsive electrostatic forces between these two negatively-charged ...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

...sequence conserved Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English...
How-to: 5 Steps to a Great Panel Discussion

Type

Blog Post

...use any slides for the event. Slides are for presentations, not discussions. I will rarely work with a ...
Tips to Make the Most of a Scientific Conference

Type

Blog Post

...themselves and then ask questions. Practice your presentation for both instances. If your poster material ...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544 become contaminated...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...
10 Basic tips for mammalian cell culture

Type

Blog Post

...their comfort zone around 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially...
Transferable Skills: Negotiation

Type

Blog Post

...more important to you and why? What are their expectations/needs and how are they different from mine? ...
Viral Vectors 101: Voltage Indicators

Type

Blog Post

...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...
Hot Plasmids: Winter 2025

Type

Blog Post

... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...
Common Injection Routes in Mice

Type

Blog Post

...finally, to expert. Figure 1. Visual representation of common injection routes in mice. Image created...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...eukaryotic organisms. Typically, what system is used is dictated by the specific type of virus you are trying to...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...sequence of DNA. Second, NgAgo target specificity is dictated by a DNA guide sequence rather than an RNA guide...

Showing: 781 - 810 of 836 results

Results per page:

Plasmids 101: The protein expression toolbox

All in a Twist: dsRNA

Giving gRNAs a Facelift - Synthetic and Beyond

Developing Lab Management Software for Biology

Gendered Innovations: Why Does Sex of the Cell Matter?

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Plasmids for Endogenous Gene Tagging in Human Cells

Adenoviral Vector Production and Troubleshooting

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Plasmids 101: Modular Cloning

CRISPR in the Clinic

Viral Vectors 101: Calcium Sensors

Antibodies 101: Choosing the Right Isotype

Hot Plasmids and Viral Preps - March 2021

5 Tips for Troubleshooting Viral Transductions

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: Terminators and PolyA signals

How-to: 5 Steps to a Great Panel Discussion

Tips to Make the Most of a Scientific Conference

AAV Vector Quality Control: Going the Extra Mile with NGS

Fluorescent Proteins 101: Fluorescent Protein Timers

Plasmids 101: Knockout/Knock-In Plasmids

10 Basic tips for mammalian cell culture

Transferable Skills: Negotiation

Viral Vectors 101: Voltage Indicators

Hot Plasmids: Winter 2025

Common Injection Routes in Mice

Production of Virus in Insect Versus Mammalian Cells

Google Forums Round Up: First Impressions of NgAgo

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