We narrowed to 838 results for: ANG
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TypeBlog Post...Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed PMID: 16547094...16547094. PubMed Central PMCID: PMC1526508. 2. H. Jiang, et al. Cytokine/Jak/Stat signaling mediates regeneration...
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Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon You! Chromatin-related genes...chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation.... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung...potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post... of blue light, iLID undergoes a conformational change allowing high affinity binding to SspB, brings ...host organism, the expression of some genes can change depending on the location. Current methods for ... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the Boards... Jennifer Tsang... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for... -
How Addgene Creates Content
TypeBlog Post...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our... -
Plasmids 101: Screens vs. Selections
TypeBlog Post... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...Developmental Editor at F1000Research. With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post... mentor met with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with...years. They helped one another navigate career changes and overcome barriers by sharing broader perspectives... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN ... -
Antibodies 101: Fab Fragments
TypeBlog Post.... Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’...:703–706. https://doi.org/10.1177/34.6.3084626 Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki... -
The time and cost required to make a plasmid
TypeBlog Post...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means... -
To Each HIS Own
TypeBlog Post...and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing resin. The protein...polyhistidine containing contaminating protein (orange). A low imidazole wash buffer disrupts the weak... -
All in a Twist: dsRNA
TypeBlog Post... hybrid molecules both exist and have a diverse range of functions within mammalian cells. Here, we will... much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...protocol modifications, and ultimately decided on the changes to implement. As with any skill, there will always...solving skills and learn to apply them flexibly in a range of contexts. As such, scientists are uniquely prepared... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry with...understanding of the physiology and pathology of the basal ganglia in movement disorders. References and Resources...