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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 843 results for: ANG

Showing: 521 - 540 of 843 results

  3. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral integration... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...

  5. How Addgene Creates Content

    Type
    Blog Post
    ...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...

  6. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic...

  7. Antibodies 101: Isotypes

    Type
    Blog Post
    ...switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ...

  8. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...

  12. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  13. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

  14. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

  15. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well...

  17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN ...

  18. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...

  19. To Each HIS Own

    Type
    Blog Post
    ...and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing resin. The protein...polyhistidine containing contaminating protein (orange). A low imidazole wash buffer disrupts the weak...

  20. All in a Twist: dsRNA

    Type
    Blog Post
    ... hybrid molecules both exist and have a diverse range of functions within mammalian cells. Here, we will... much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins...
Showing: 521 - 540 of 843 results