We narrowed to 835 results for: ANG

...bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...

...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...

... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic...

...switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ...

...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...

...transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral integration... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...

...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well...

... mentor met with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with...years. They helped one another navigate career changes and overcome barriers by sharing broader perspectives...

...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN ...

...abilities is that its fluorescence emission spectra changes when it goes from a more neutral pH compartment...emission spectra. Component Excitation Emission pH Range DsRed.T3 488, 543*, 568 nm 587 nm ~4.9 - ...

...to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the Addgene... Angela Abitua...

...parts in the micromolar or nanomolar concentration range. Reactions to happen under lots of conditions ...from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands HaloTag Reversible binding of...

....  Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’...:703–706. https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki...

...otherwise it would be a lot quicker to pick up new languages!) Jargon has a place in science communication,...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

...protocol modifications, and ultimately decided on the changes to implement. As with any skill, there will always...solving skills and learn to apply them flexibly in a range of contexts. As such, scientists are uniquely prepared...

...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...

...and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing resin. The protein...polyhistidine containing contaminating protein (orange). A low imidazole wash buffer disrupts the weak...

... hybrid molecules both exist and have a diverse range of functions within mammalian cells. Here, we will... much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins...

...monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry with...understanding of the physiology and pathology of the basal ganglia in movement disorders.   References and Resources...

...al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert et al., 2014)...: 24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using...