We narrowed to 648 results for: ATO

1. Cpf1 Update: Comparison to Cas9 and NgAgo

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   Blog Post

   ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...

2. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...the work I agreed upon (just as I would in my laboratory work). Committee members have to work professionally...

3. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ...trying to explore the links between genetics and anatomy, which are not yet fully understood. We attempt...

4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   ...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...

5. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

6. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

7. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

8. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

9. Plasmids 101: Protein Expression

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   Blog Post

   ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...

10. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold. With an NGG PAM, xCas9...

11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

12. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...

13. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post

    ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...

14. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ...Blouin-Tavel, V., Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R.,...

15. Transferable Skills Guide: Managing a Team

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    Blog Post

    ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

17. Academic vs. Industry Postdocs

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    Blog Post

    ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

19. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

20. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...