...engineer and scientist, I like to envision that my research will have a direct impact on healthcare and the...result, I have occasionally pondered translating my research into a startup company. However, like many like-minded...incorporated into Novophage Therapeutics, Inc. in March 2009 and tested in the “market” by competing in ...

... podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what... Yeast Transcription Factors Podcast Segment Researchers from the El-Samad lab recently deposited plasmids...that this level of control will enable future researchers to better simulate physiological gene expression...

...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...low resolution and can’t be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins...CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic locus,...

...Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...this process is not fully understood, a team of researchers lead by Takayuki Kogure (Kogure et al., 2011)...

...out plenty of roles just the way they are, the research antibodies in your refrigerator often need a little...are kind of hard to see. To solve this issue, researchers conjugate various labels to antibodies that produce...conjugation, which is commonly used for labeling research antibodies. Lysine residues are found throughout...

...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method.  Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...

... School of Medicine and a researcher at the Yerkes National Primate Research Center. Using a combination...combination of in vivo and anatomical techniques, her research aims to gain a deeper understanding of the physiology...

...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...editing efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor,...

...as part of its natural viral life-cycle, and researchers have adapted the components for use in genome...-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics...parallel. Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

... AAV vectors evade host immunity. While basic research and other ongoing clinical trials support the ...Silva Feelixge. Harshana S De Silva Feelixge is a researcher whose work has focused on gene therapy for viral... She is particularly passionate about HIV cure research and science communication.     References 1. Gaspar...

...again reminded of the extent that C. elegans researchers embrace open science and share resources and ...developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed PMID: 23172286...for 3′ UTR biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455...

...provide researchers with useful and adaptable tools to perform basic and preclinical research. For scientists...Inbred and outbred strains Mouse strains used in research can fall under one of two broad classifications...

... doesn’t mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went...choice, but by circumstance. She was working as a research and technical assistant, when her life changed...often under a lot of pressure to publish their research and are not so keen on sharing their results in...

...only for translational research, such as gene therapy, but also basic research. To improve cell-type specificity...valuable tools for basic as well as translational research for gene therapy. The Roska lab hopes to design...

... the reagents available at Addgene come from researchers, and the vast majority of our plasmids and pooled...it, there’s a reason nearly 70% of all internet searches are done via Google. The “Let me Google that for...questions, consider using Google Scholar, which searches scientific articles, patents, and even some doctoral...

...CRISPR toolbox. When Cpf1 was first discovered, researchers wondered if it could overcome some of Cas9’s ...templates should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ...possibility has not been experimentally confirmed. Researchers have also not yet characterized the potential...

...working at the Association for Research in Vision and Ophthalmology Research (ARVO). Hopefully sharing my... in closer connection with other leaders in my research field should I decide to remain here.  In a time...

... pretty good. We started doing extracurricular research on some of them to see how feasible they were,... for us? After about a month of discussion and research and lots of conversation, we hit upon the idea... They invested in us this summer – $30,000 for research and another $30,000 in mentorship and business...

...d like to modify. To tackle the PAM problem, researchers have engineered alternative Cas9s binding distinct...this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants with alternative...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

... and what is their background relative to your research? Tailor your language to your audience so that...presenting at the American Association for Cancer Research Annual Meeting, you might consider including more...group who presented on a topic related to your research? Find a way to get in contact with the presenter...