We narrowed to 644 results for: CAR
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TypeProtocol...move your microscope around the lab, be sure to carry it with two hands - one hand supporting the base...base and the other holding the arm - or use a cart. Revolve the nosepiece so that the lowest power objective...objective. Take your eyes away from the eyepiece and carefully revolve the nosepiece so that the next objective...will notice that the objectives get larger. Be careful to avoid hitting your sample with the objective...laboratory instrument, you should be sure to take care of your microscope. Bumps, scratches, and dust can...
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Protocol - How to Run an Agarose Gel
TypeProtocol...the second stop and carefully raise the pipette straight out of the buffer. Carefully load your samples ...occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling can occur. Pro-Tip It...not added EtBr to the gel in the first place. Carefully load a molecular weight ladder into the first ...the electrodes from the power source, and then carefully remove the gel from the gel box. Optional: If ... -
A Look at Addgene's QC Process
TypeBlog Post...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources Additional... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post... active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting, unlike other well-characterized... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post... developed a second set: The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...the virus so that it only enters and delivers it cargo to certain cell types. Botond Roska’s lab at the... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...containing structural genes and a transfer plasmid carrying the transfer gene. By dividing the viral elements... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette... -
Rabies and Neuronal Tracing
TypeBlog Post...efficiently enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...cells. What’s more, G-baToN can be used to bring cargo, such as protein or DNA, to manipulate function ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... to make sure the deposit agreement/MTA is taken care of and, once we have all of your plasmid data, we... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post... - they can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different AAV serotypes have preferences... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...for your genes of interest, your F1 mice will be carriers at those alleles. Some alleles, such as Cre, function... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...generally less common and will stand out; however, be careful not to hang your poster too low. If it is pinned... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...improved transcript life, and so should be handled with care (4). For this reason, viral vectors often employ... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post... plasmid collection Browse the RESOLUTE solute carriers collection Find other COVID-19 and Coronavirus... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...interest. They created a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...alter for a purpose and To direct the professional career of. Like everyone else I mostly learned through...