We narrowed to 641 results for: CAR

1. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette...

2. Rabies and Neuronal Tracing

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   ...efficiently enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged...

3. Plasmids 101: Terminators and PolyA signals

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   ...improved transcript life, and so should be handled with care (4). For this reason, viral vectors often employ...

4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ... to make sure the deposit agreement/MTA is taken care of and, once we have all of your plasmid data, we...

5. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   ...generally less common and will stand out; however, be careful not to hang your poster too low. If it is pinned...

6. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ... - they can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different AAV serotypes have preferences...

7. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...for your genes of interest, your F1 mice will be carriers at those alleles. Some alleles, such as Cre, function...

8. Hot Plasmids and Viral Preps - January 2021

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   ... active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting, unlike other well-characterized...

9. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ... developed a second set: The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA...

10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...the virus so that it only enters and delivers it cargo to certain cell types.  Botond Roska’s lab at the...

11. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies...

12. Hot Plasmids and Viral Preps - March 2021

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    ...cells. What’s more, G-baToN can be used to bring cargo, such as protein or DNA, to manipulate function ...

13. Progress Towards a PAM-Free CRISPR

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    ...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat...

14. A Look at Addgene's QC Process

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    ...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources Additional...

15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...interest. They created a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial...

16. Transferable Skills Guide: Managing a Team

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    ...alter for a purpose and To direct the professional career of. Like everyone else I mostly learned through...

17. Google Forums Round Up: First Impressions of NgAgo

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    ...achieve greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide,...

18. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...could be forming dimers in cells. Why should you care? Three simple ways a dimeric FP could ruin your ...

19. 3D Printing Meets CRISPR Cas9

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    ...now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more accurately...

20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...your mutation. To maintain stable lines of flies carrying a specific mutation, Drosophila geneticists use...