We narrowed to 802 results for: Eng
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TypeBlog Post...abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the...
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Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...time. Searching for literature My go-to search engines for finding scientific literature are Google Scholar...relevant. Harness the power of the Google search engine and sign up for Google Scholar alerts. Based on... -
How to Prepare for an Industry Interview
TypeBlog Post... time out of their day to talk with you, so be engaged with their conversation. Send thank you emails ...carefully. And even if you don’t have technical challenges, your interviewers might. You’ll probably still... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...Therefore they make excellent tools for genetic engineering. There are several different viral vectors to...our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ... -
Rabies and Neuronal Tracing
TypeBlog Post...and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools...Watabe-Uchida et al., 2012). They could also quantify the strength of the inputs by counting the monosynaptic inputs... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...decaffeination operon podcast segment With the help of engineered microbes, used coffee grounds could be economically...generalized to measure phosphatase activity, second messenger activity, and protein-protein interactions. We... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial traits...Gateway cloning. In addition, scientists have engineered entirely new types of plasmids without clear ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure... -
Viral Vectors 101: Virus Safety
TypeBlog Post...safety features When viruses were first being engineered as biological tools, thought was given on how...replication of virus and its infectivity in check. Engineering controls, safety systems that are built into ... -
Hot Plasmids February 2024
TypeBlog Post...here! Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...Researchers in the Gradinaru lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...Yongsub Kim’s lab engineered prime editors to expand their PAM flexibility. Find the engineered prime editors... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves....2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated... -
Plasmids 101: FLEx Vectors
TypeBlog Post...Hongkui Zeng Additional Resrouces on the Addgene Blog Read about Other Means of Genome Engineering Using... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...real-time, posing a huge challenge to studying epigenetic changes over time. This challenge was difficult to ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...Makerspace is composed of computer scientists, engineers, and biologists working together on a variety ... case. Funding Makerspaces One of the main challenges when starting a new project at a makerspace is... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...experiments, and prepared your reagents. You’re going to engineer the best thing since CRISPR; you are ready to ...technologies currently available, you now face the challenge of trying to figure out what system might work...