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We narrowed to 1,148 results for: Gene

Showing: 521 - 540 of 1148 results

  1. All in a Twist: dsRNA

    Type
    Blog Post
    ...dissociate when gene transcription is complete. However, transcription of thousands of genes is constantly...store their genetic information. All of these viruses have one thing in common – they can generate dsRNA as... Here, we will cover how these structures are generated, why it’s important for cells to sense their formation...inherently double stranded, while ssRNA viruses can generate dsRNA intermediates as part of the viral replication...as long/short interspersed nuclear elements can generate dsRNA by bidirectional transcription or as inverted...inverted repeats. These elements are almost always epigenetically silenced, but dysregulation of these systems... ionizing radiation, can also result in dsRNA generation. While these molecules are clearly associated...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...editing, this epigenetic strategy doesn’t induce DNA damage or permanently alter a gene product; it simply...on Prime-editing-Assisted Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes...magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that...with ribonucleoprotein cargo. eVLPs contain no genetic material, which ensures the editor will only be...

  3. The 12 Days of CRISPR

    Type
    Blog Post
    ...cells🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic... of CRISPR, Addgene gave to me: Cas-E-le-ven! (Cas11)🎵 On the 10th day of CRISPR, Addgene gave to me:... day of CRISPR, Addgene gave to me: RADAR from Gao🎵On the 8th day of CRISPR, Addgene gave to me: Functional...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to ...let's get started!  On the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function...Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing (CRISPEY!)🎵 On the 7th...7th day of CRISPR, Addgene gave to me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Alkaline phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy...Pho8Δ60 in the vacuole, where it is cleaved to generate an active enzyme. Pho8 phosphatase activity or.... cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful for...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...that there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002). G proteins...2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520... on Addgene.org Check out Addgene's chemogenetics guide Find chemogenetics plasmids for your research...cell type. Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors...neurons and specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small...are ideal candidates for use in chemogenetics. To create chemogenetics receptors, GPCRs were first mutated... about chemogenetics and a concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics...

  6. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...studies for various categories, including CRISPR gene-editing, the foundations of synthetic biology, DNA... the editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon...

  7. Sequencing Options for CRISPR Genotyping

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    Blog Post
    ...triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 (2015): 407-415. PubMed PMID: 25566793...off-target mutation and not an edit in the target gene. The validation process, also known as CRISPR genotyping.... 12. Osborn, Mark J., et al. "Evaluation of TCR gene editing achieved by TALENs, CRISPR/Cas9, and megaTAL...as the field seeks to generate reproducible, quality data on the role of genetic networks in biological... the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse... CRISPR experiments, many are turning to next-generation sequencing options over older assays. Biased... therapy. Low frequency off-target effects may generate irreproducible data in a research setting, but...

  8. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed for the Fragmid...plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the vector file....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...select the components they want for each module, generate the vector file, and quickly clone their vector...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...

  9. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...you attempt to verify the starting methionine of a gene but the blastx results start the alignment at a ...unlikely to have an exact entry for your favorite gene fused to an epitope tag or fusion protein. If your... our Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...

  10. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells us about... share on our blog, please reach out to news@addgene.org - we'd love to hear from you!       ...

  11. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Sana on LinkedIn.   References 1. Yin, R., et al. "Gene expression profiling analysis of bisphenol A-induced...induces cytoskeletal disorganization, apoptosis, and gene expression disregulation on HeLa cells." Journal...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...Neuroscientist's View Control Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression...selectively infect a genetically defined cell population. While the EnvA genetic sequences are supplied...combination with molecular tools (e.g., optogenetic or chemogenetic receptors) enables complex control of...enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful...individual neurons they infect. However, they generally are not transmitted to adjacent neurons via synapses...of the fact that RABV is an enveloped virus. In general, viral envelopes dictate infection of target cells...

  13. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post
    ...years, but lay readers may be less familiar with gene drives and their ramifications. Readers unfamiliar...in Doug Prasher, who originally isolated the GFP gene but was not included amongst the Nobel prize winners...time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with ...contributed to the steadily increasing distrust the general public has in science and scientists (as discussed...replace scientists themselves.  The sections on optogenetics and CRISPR of course address some of the ethical...has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar with the role...I discussed The State of Science with the five Addgene colleagues who read it with me, the consistent ...

  14. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ... selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic ...Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...aren’t working, you can check if virus is being generated by your transfected packaging cells. This check...and transduction. Once you’ve harvested, it’s generally a good idea to titer your virus. Getting an accurate...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Medicine. He is interested in everything related to gene expression, particularly at the RNA level. He maintains...fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics” ...flux, etc). The most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...proteins interact. Oligomerization: The first generations of FPs were prone to oligomerize. This may affect...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  16. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make COVID-19 ... 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500...whether you are a depositor or not. We will have Addgene trivia (with prizes!), polls, and shout outs, and... and we’ll be featuring the work of the Addgene community. Perhaps you’ll find plasmids and new tools ...We’ll also take a look back on some fascinating Addgene history that you might not have known about. Here's...Thursday: Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude...

  17. Tips for arabidopsis transformation

    Type
    Blog Post
    ...transcriptional and translational dynamics of genes and proteins in living plants, to complementing mutant phenotypes...potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop....simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org Find plant... transformation. There are many motivations to generate transgenic Arabidopsis, from studying transcriptional... floral drip or dip transformation method. The general steps for this method include: Cloning and transforming...Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate...appropriate expression vector is the first step in generating transgenic Arabidopsis. There are a bunch of ...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter, and ...genomic insertions and/or deletions, to multiplexed gene knockouts (Vo et al., 2020), to multi-copy genomic...a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner), as a method...Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between... CRISPR-Cas, these systems represent the next generation of tools for efficient and programmable insertions... specifically recognized during transposition, genetic cargos must be flanked by the L and R ends - thus... at programmable insertions of even very large genetic cargo, at one or multiple desired genomic loci....

  19. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    .... With the ability of viral vectors to fine tune gene expression temporally and spatially, exciting times... insights into the complex relationship between genes, neurons, and behavior. There are several other ...Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these existing findings...are combining genetic manipulations with the forced swim test understanding the genetic background of ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...behavior. We’ve previously discussed some of the general considerations for performing behavioral tests,...within the LC of mice. When those neurons are chemogenetically activated, norepinephrine release causes a...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Right: T1 tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron...mouse monoclonal antibodies targeting the human genes and are recommended for use in immunohistochemistry...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots...applicable to dicot species. In addition, they generated offspring tomato plants that inherited the genomic...single-molecule fluorescence techniques. After generating a series of single- and double-tagged constructs...
Showing: 521 - 540 of 1148 results