Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,145 results for: Gene

Showing: 521 - 540 of 1145 results

  1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on....  Blugene gets a make-over You may also have noticed another big update on the homepage. Blugene, our... ways, we realized that Blugene needed the same thoughtful treatment. Blugene has been with us since 2011...might notice Blugene looking sad, especially if an experiment doesn’t go as planned. Blugene also has full...

  3. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...For example, this is an experiment using the chemogenetic tool hM4D(Gi) driven by a GFAP promoter in rat... rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

  4. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check... Institutions that Deposit their Plasmids with Addgene ...

  5. Synthetic Biology - Algal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in algae. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  6. Synthetic Biology - Bacterial

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in bacteria. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....to see added to this list, please email help@addgene.org . Synthetic Biology Plasmids Search the table...

  8. Synthetic Biology - Fungal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in fungus. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  9. Synthetic Biology - Plant

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in plants. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. Synthetic Biology - Yeast

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in yeast. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  11. AAV Packaged on Request

    Type
    Collection
    ...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian... more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources Viral Vector...recommended for research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of seven serotypes and... 50 × 100 µL aliquots 5.0 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL...more about AAV serotypes and tissue tropism on Addgene's blog . All-in-One Pricing The price for our Packaged...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    ....1216880109 Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human...kDas up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame to the protein of interest... protease is situated between the degron and the gene of interest and the degron domain is terminal. Between...sequence (7 kDa). This system also requires less genetic engineering than some of the other systems reviewed... leakiness associated with the system, but the general concept of the system has remained the same. An...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange...the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, after transforming...3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such lab is...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used...recombination events: The plasmid carrying the desired genetic modification integrates into the genome (Figure...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series...

  14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ... the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things...Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose...to find plasmids matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker:...Resistance: Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ...search, we’ve also revamped our homepage and mascot Blugene (check back on December 5 to learn more about this...

  15. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...qPCR is used in a variety of applications including gene expression profiling, studying copy number variation... is also used in high throughput SNP genotyping, gene deletion analysis, and RNA detection.  A summary...starting template DNA concentration Gene expression profiling Copy number variations Quantify RNA levels...Disease or pathogen identification SNP genotyping Gene deletion Analysis RNA detection     References...in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...

  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...including: generating single or multi-gene knockouts in a wide variety of cell types, gene editing using...efficiency, requires specialized equipment, such as a gene gun. Depending on the nature of the experiment, ... CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing." Journal of the American Chemical Society...using homology directed repair (HDR), and generating large genomic deletions. What are the advantages of...and specificity is a concern, such as knockout generation or homologous recombination.  However, experiments...step in this type of CRISPR experiment is the generation of the RNP complex.  While one option is to purchase...in vitro transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...cell lines with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with...cDNA is co-transfected at a favorable ratio to the gene of interest (or, if your protein is fused with a...with different levels of expression of our gene of interest. Advantages of the agarose cloning method ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...-well cell plate to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...

  18. CRISPR Cheat Sheet

    Type
    Blog Post
    ...expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple genes in a cell population...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. ...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated ...example, choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate...be okay if you are introducing a selection marker gene. The homology arms should be no more than 100 bp...for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper ...Winderbaum, J., Nickoloff, J. A., & Jasin, M. (1998). Gene Conversion Tracts from Double-Strand Break Repair...Additional resources on Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software...

  20. Synthetic Biology - Mammalian

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in mammalian systems. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 521 - 540 of 1145 results