We narrowed to 527 results for: HA tag

1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   Blog Post

   ...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...genome-wide screens.   Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen...

2. Twenty Years of Sharing: Expanding Accessibility

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   Blog Post

   ...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at room temperature...

3. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   Blog Post

   ...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...introduction of an intended mutation while taking advantage of the improved efficiency provided by blocking...

4. CRISPR 101: Validating Your Genome Edit

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   Blog Post

   ...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...sequence and other regions of the genome. The main advantage of NGS is that it allows you to simultaneously...

5. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   ...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...itself, like laser type and power, acquisition voltage, the concentration of the fluorophore, and whether...bd-spectrum-viewer.   But what is the real-life advantage of a brighter fluorophore? Strong brightness makes...

6. Finding Your Science Policy Path

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   Blog Post

   ...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple place to...an account and start engaging with the #scipol hashtag. Often there will be posts about virtual meet ups...

7. Trimmer Lab NeuroMab Collection

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   Collection

   .../9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [N77...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful for... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin... Mouse Mouse IgG2a 177526 Anti-Synaptotagmin-7 [N275/14R] Synaptotagmin-7 Mouse Mouse IgG2a 177527 Anti-Lgi1... Human Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK...