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We narrowed to 844 results for: Met;

Showing: 521 - 540 of 844 results
  1. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ...foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and eat, and...vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are...
  2. Some Like it Hot: Thermostable GeoCas9

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    Blog Post
    ...? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know in the ...Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering   Interested in...
  3. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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    Blog Post
    ...peptides sequences into these variable loops is one method for changing a capsid’s tropism.  cap also encodes...the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org...
  4. Fostering communication in remote collaborations

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    Blog Post
    ...scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog...
  5. Plasmids 101: What is a plasmid?

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    Blog Post
    ...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...
  6. 10 Ways to Share Your Science!

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    Blog Post
    ...ask and answer questions and hopefully discover something new. Science involves success and, more often,...not just the details of your science but also something about how science really works. 2)   Read science...
  7. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...people) -> How can you make a decision about something like this without sufficient data? Starting to...
  8. Plasmids 101: TOPO Cloning

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    Blog Post
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang...
  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    Blog Post
    ...limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator system...Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127. PubMed...
  10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...(2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat... of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276...
  11. Intro to Our Summer SciComm Series!

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    Blog Post
    ...communication itself, not the communication method, that determines if something is scientific or science communication...
  12. RUBY-Red Siliques

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    Blog Post
    ... instead, which can’t be done if your selection method kills the transgene free individual. For example...studying large samples. RUBY-Red But recently, something new has been added to the palette. RUBY is a bright...
  13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...
  14. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...Isotype matters when constraining using the indirect method since a secondary antibody reactive to a certain... not be used for multiplexed indirect staining methods since the secondary antibody used will react to...
Showing: 521 - 540 of 844 results