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Showing: 521 - 540 of 840 results
  1. Plasmids 101: What is a plasmid?

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    Blog Post
    ...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...
  2. Some Like it Hot: Thermostable GeoCas9

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    Blog Post
    ...? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know in the ...Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering   Interested in...
  3. 10 Ways to Share Your Science!

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    Blog Post
    ...ask and answer questions and hopefully discover something new. Science involves success and, more often,...not just the details of your science but also something about how science really works. 2)   Read science...
  4. Plasmids 101: TOPO Cloning

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    Blog Post
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang...
  5. Intro to Our Summer SciComm Series!

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    Blog Post
    ...communication itself, not the communication method, that determines if something is scientific or science communication...
  6. RUBY-Red Siliques

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    Blog Post
    ... instead, which can’t be done if your selection method kills the transgene free individual. For example...studying large samples. RUBY-Red But recently, something new has been added to the palette. RUBY is a bright...
  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...(2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat... of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276...
  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...people) -> How can you make a decision about something like this without sufficient data? Starting to...
  9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...
  10. Antibodies 101: Chimeric Antibodies

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    ...Isotype matters when constraining using the indirect method since a secondary antibody reactive to a certain... not be used for multiplexed indirect staining methods since the secondary antibody used will react to...
  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ...limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator system...Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127. PubMed...
  12. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...
  13. Another Pathway into Cells: iTOP

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    Blog Post
    ...description of the lab’s iTOP protein-only transduction method and its potential applications to CRISPR/Cas9 genome...osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies showed that...
  14. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...addressing these important questions that the scientific method cannot address. Listen to Our Podcast Interview...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. ...
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